Recombinant Human IL-17RA/IL-17R Avi-tag His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11234

R&D Systems, part of Bio-Techne
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AVI11234-050
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Key Product Details

Source

HEK293

Accession #

Q96F46.2

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-17RA/IL-17R Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-17RA/IL-17R protein
Human IL-17RA
(Leu33-Trp320)
Accession # Q96F46.2		 Avi-tag 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu33

Predicted Molecular Mass

38 kDa

SDS-PAGE

63-69 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human IL-17RA/IL-17R Avi-tag His-tag binds to IL-17A (Human Cell-expressed) Protein (Catalog # 7955-IL) with an ED50 of 3.00-30.0 ng/mL.

Scientific Data Images for Recombinant Human IL-17RA/IL-17R Avi-tag His-tag Protein, CF

Recombinant Human IL-17RA/IL-17R Avi-tag His-tag Protein Binding Activity.

Recombinant Human IL-17RA/IL-17R Avi-tag His-tag Protein (Catalog # AVI11234) binds to IL-17A (Human Cell-expressed) Protein (7955-IL) with an ED50 of 3.00-30.0 ng/mL.

Recombinant Human IL-17RA/IL-17R Avi-tag His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL-17RA/IL-17R Avi-tag His-tag Protein (Catalog # AVI11234) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 63-69 kDa, under reducing conditions.

Formulation, Preparation and Storage

AVI11234
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RA/IL-17R

IL-17 R, also known as IL-17 RA, is a 120 kDa type I transmembrane glycoprotein protein that plays a central role in inflammatory responses (1-3). Mature human IL‑17 R consists of a 288 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 525 aa cytoplasmic domain (4). The cytoplasmic domain contains a region homologous to the TIR domain of the TLR/IL-1 R family (5). Human IL-17 R shares 72% aa sequence identity with mouse and rat  IL-17 R.  Within the extracellular domain, it shares 18%-25% sequence identity with human IL-17 RB, C, D, and E. While the expression of IL-17 is restricted to activated T cells, IL-17 R exhibits a broad tissue distribution (4). Even in the absence of ligand, IL-17 R exists on the cell surface as a multimer (6). IL-17 R can bind IL-17 but must associate with IL-17 RC to transduce signals (7, 8). Interestingly, human IL-17 R does not appear to form productive complexes with mouse IL-17 RC (8). The IL-17 R can also signal in response to IL-17F (9). IL-17 R ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple pro‑inflammatory chemokines (4, 7, 9, 10). IL-17A and IL-17F synergize with TNF-alpha in the induction of CXCL1, G-CSF, and IL-6 (9, 11). This effect requires the presence of both TNF RI and TNF RII (9). IL-17 interactions with IL-17 R also inhibit the TNF-alpha induced up-regulation of fibroblast CCL5 and VCAM-1 (11). CCL5 and VCAM-1 induced effects are differentially sensitive to blockade with IL-17 R specific antibodies, suggesting that IL-17 R triggers divergent intracellular signals (11). In vivo, IL‑17 R activity is important for increased generation of neutrophils and their recruitment to sites of inflammation (10, 12, 13). IL-17 R is required for host defense against microbial infection and for the progression of arthritis from inflammation to destructive joint erosion (10, 13). Our Avi-tag Biotinylated human IL-17R Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Iwakura, Y. and H. Ishigame (2006) J. Clin. Invest. 116:1218.
  2. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  4. Yao, Z. et al. (1995) Immunity 3:811.
  5. Novatchkova, M. et al. (2003) Trends Biochem. Sci. 28:226.
  6. Kramer, J.M. et al. (2006) J. Immunol. 176:711.
  7. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  8. Toy, D. et al. (2006) J. Immunol. 177:36.
  9. McAllister, F. et al. (2005) J. Immunol. 175:404.
  10. Ye, P. et al. (2001) J. Exp. Med. 194:519.
  11. Schnyder, B. et al. (2005) Cytokine 31:191.
  12. Tan, W. et al. (2006) J. Immunol. 176:6186.
  13. Lubberts, E. et al. (2005) J. Immunol. 175:3360.

Long Name

Interleukin 17 Receptor

Alternate Names

CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Gene Symbol

IL17RA

UniProt

Q96F46.2

Additional IL-17RA/IL-17R Products

Product Documents for Recombinant Human IL-17RA/IL-17R Avi-tag His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-17RA/IL-17R Avi-tag His-tag Protein, CF

For research use only

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