Recombinant Human IL-17RA/IL-17R Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 177-IR
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human IL-17 RA/IL-17 R protein
| Human IL-17 RA/IL-17 R (Leu33-Trp 320) Accession # Q96F46 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu33
Predicted Molecular Mass
60 kDa (monomer)
SDS-PAGE
100 kDa, reducing conditions
Activity
Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 0.03-0.1 µg/mL in the presence of 10 ng/mL recombinant human IL‑17.
The ED50 for this effect is 0.03-0.1 µg/mL in the presence of 10 ng/mL recombinant human IL‑17.
Formulation, Preparation and Storage
177-IR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-17RA/IL-17R
Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL‑17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy‑proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL‑17 includes activation of NF‑ kappaB and JNK, and require TNF receptor‑associated factors 6 (TRAF6) in the signaling pathway.
References
- Yao, Z. et al. (1997) Cytokine 9:794.
- Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
Long Name
Interleukin 17 Receptor
Alternate Names
CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA
Entrez Gene IDs
Gene Symbol
IL17RA
UniProt
Additional IL-17RA/IL-17R Products
Product Documents for Recombinant Human IL-17RA/IL-17R Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human IL-17RA/IL-17R Fc Chimera Protein, CF
For research use only
Loading...
Loading...
Loading...