Recombinant Human IL-19 (NS0-expressed) Protein

R&D Systems, part of Bio-Techne | Catalog # 9286-IL

R&D Systems, part of Bio-Techne
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Carrier Free
9286-IL-025/CF
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With Carrier
9286-IL-025
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Key Product Details

Source

NS0

Accession #

AAN40906

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-19 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-19 protein
Leu25-Ala177

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

18 kDa

SDS-PAGE

20-30 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL-20 R alpha and human IL-20 R beta.
The ED50 for this effect is 0.1-0.6 ng/mL.

Scientific Data Images for Recombinant Human IL-19 (NS0-expressed) Protein

Recombinant Human IL-19 (NS0-expressed) Protein Bioactivity

Recombinant Human IL-19 (NS0-expressed) Protein Bioactivity

Recombinant Human IL-19 (Catalog # 9286-IL) induces proliferation of BaF3 mouse pro-B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 0.1-0.6 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9286-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 9286-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-19

Interleukin 19 (IL-19) is a member of the IL-10 family of class II alpha-helical cytokines that contains two groups, a viral homolog and cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R beta as a signal transducing receptor (IL-10, IL-22, and IL-26), and one which uses IL-20 R beta as a signal transducing receptor (IL-19, IL-20, and IL-24) (1). The 153 amino acid (aa) mature human IL-19 is secreted as a glycosylated 35-45 kDa monomer (2-4). It shares approximately 70% aa sequence identity with mouse and rat IL-19. Human IL-19 may utilize an alternate start site that adds 38 aa to the N-terminus. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (2). IL-19 is expressed on activated keratinocytes, monocytes, and B cells (3, 5, 6). It binds a receptor complex consisting of IL-20 R alpha and IL-20 R beta (3, 7, 8). This receptor complex is also shared by IL-20 and IL-24 (9, 10). It has been reported that IL-19 both will and will not induce IL-6 and TNF-alpha production by monocytes (2, 11). It does, however, drive T helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (2, 11, 12).

References

  1. Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
  2. Liao, Y-C. et al. (2002) J. Immunol. 169:4288.
  3. Gallagher, G. et al. (2000) Genes Immun. 1:442.
  4. Pletnev, S. et al. (2003) Biochemistry 42:12617.
  5. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
  6. Wolk, K. et al. (2002) J. Immunol. 168:5397.
  7. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  8. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517.
  9. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
  10. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
  11. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
  12. Liao, S-C. et al. (2004) J. Immunol. 173:6712.

Long Name

Interleukin 19

Alternate Names

IL19

Entrez Gene IDs

29949 (Human); 329244 (Mouse)

Gene Symbol

IL19

UniProt

AAN40906

Additional IL-19 Products

Product Documents for Recombinant Human IL-19 (NS0-expressed) Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 9286-IL (with carrier)
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Product Specific Notices for Recombinant Human IL-19 (NS0-expressed) Protein

For research use only

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