Recombinant Human IL-22BP Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI1087

Biotinylated
R&D Systems, part of Bio-Techne
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AVI1087-025
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Key Product Details

Source

HEK293

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-22BP Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-22BP protein
Human IL-22BP
(Thr22-Pro231)
Accession # NP_851826.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr22

Predicted Molecular Mass

53 kDa

SDS-PAGE

73-82 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-22 Protein (Catalog # 782-IL) is coated at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human IL‑22BP Fc Chimera Avi-tag (Catalog # AVI1087) binds with an ED50 of 50.0-400 ng/mL.

Scientific Data Images for Recombinant Human IL-22BP Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human IL‑22BP Fc Chimera Avi-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-22 Protein (782-IL) is coated at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human IL‑22BP Fc Chimera Avi-tag (Catalog # AVI1087) binds with an ED50 of 50.0-400 ng/mL.

Recombinant Human IL-22BP Fc Chimera Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human IL-22BP Fc Chimera Avi-tag Protein (Catalog # AVI1087) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-82 kDa and 146-164 kDa, respectively.

Formulation, Preparation and Storage

AVI1087
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: IL-22BP

Interleukin 22 binding protein (IL-22BP), also known as CRF210, CRF2X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10Rb. IL-10Rb is also a component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29 (1, 2). IL-22BP blocks the interaction of IL-22 with IL-22 R, preventing IL-22 induced production of reactive oxygen species, IL6, IL-10, and TNFa (3-8). In vivo, it regulates the proinflammatory effe-cts of IL-22 (e.g. neutrophil infiltration) but not of IL-10 (7). Mouse IL-22BP can neutralize the bioactivity of both mouse and human IL-22 (6). IL-22BP is produced by dendritic cells (DC), epithelial cells, activated B cells, and activated monocytes (3, 6, 9, 10). It is constitutively expressed by DC but is down regulated during local inflammation and in response to tissue damage (11-13). IL-22BP is critical for limiting IL-22 induced epithelial cell proliferation during wound healing, and its deficiency can enable uncontrolled proliferation and enhance tumor development (12). Mature human IL-22BP contains two Fibronectin type-III domains (4, 6). Alternative splicing generates additional isoforms that contain a 32 amino acids (aa) insertion in the first FnIII domain and may also be truncated within the second Fc-III domain (3, 4, 14). Human IL-22BP without the 32 aa insertion shares 68% and 73% amino acid (aa) sequence identity with mouse and rat IL-22BP, respectively. Our Avi-tag Biotinylated Recombinant Human IL‑22BP features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Lim, C. and R. Savan (2014) Cytokine Growth Factor Rev. 25:257.
  2. Mizoguchi, A. (2012) Inflamm. Bowel Dis. 18:1777.
  3. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
  4. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  5. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  6. Wei, C.-C. et al. (2003) Genes Immun. 4:204.
  7. Weber, G.F. et al. (2007) Infect. Immun. 75:1690.
  8. Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.
  9. Lecart, S. et al. (2002) Int. Immunol. 14:1351.
  10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  11. Wolk, K. et al. (2007) J. Immunol. 178:5973.
  12. Huber, S. et al. (2012) Nature 491:259.
  13. Martin, J.C.J. et al. (2014) Mucosal Immunol. 7:101.
  14. Dumoutier, L. et al. (2001) J. Immunol. 166:7090.

Long Name

Interleukin 22 Binding Protein

Alternate Names

CRF2-10, CRF2-X, IL-22 RA2, IL22BP, IL22RA2

Entrez Gene IDs

116379 (Human); 237310 (Mouse)

Gene Symbol

IL22RA2

Additional IL-22BP Products

Product Documents for Recombinant Human IL-22BP Fc Chimera Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-22BP Fc Chimera Avi-tag Protein, CF

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