Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6834-ILB/CF
Key Product Details
Product Specifications
Source
E. coli-derived human IL-36 beta/IL-1F8 protein
Arg5-Glu157
Arg5-Glu157
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg5
Predicted Molecular Mass
17 kDa
SDS-PAGE
17 kDa, reducing conditions
Activity
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells.
The ED50 for this effect is 0.8-4.8 ng/mL.
The ED50 for this effect is 0.8-4.8 ng/mL.
Scientific Data Images for Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF
Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein Bioactivity
Recombinant Human IL-36 beta/IL-1F8 (Catalog # 6834-ILB/CF) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8-4.8 ng/mL.Formulation, Preparation and Storage
6834-ILB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-36 beta/IL-1F8
IL-18, IL-36Ra/IL-1F5, IL-36 alpha/IL-1F6, IL-37/IL-1F7, IL-36 gamma/IL-1F9, and IL-1F10 (1, 2). IL-1 family cytokines are characterized by a 12 beta-stranded beta-trefoil configuration and share up to 50% amino acid (aa) sequence identity. The 157 aa human IL-36 beta does not have a canonical signal peptide or prosegment and is expressed as two isoforms that differ in their C-terminal 70 aa. IL-36 beta1 lacks four of the conserved beta-strands common to the IL-1 family (3). Human IL-36 beta2 shares 62%, 67%, 63% and 59% aa identity with the most similar isoforms of mouse, canine, bovine and equine IL-36 beta, respectively (4). IL-36 beta is expressed by keratinocytes, naïve CD4+ T cells, neurons, and glia (5-7). It is up-regulated in keratinocytes and synovial fibroblasts by inflammatory stimulation and in psoriatic lesions (5, 8, 9). IL-36 beta promotes inflammatory responses by enhancing the activation and Th1 polarization of dendritic cells and T cells (7, 10, 11). It also enhances the production of multiple pro-inflammatory cytokines, chemokines, and anti-bacterial defensin peptides by keratinocytes, synovial fibroblasts, and articular chondrocytes (5, 8-10). IL-36 proteins exert their bioactivity through a receptor complex that contains IL-1 Rrp2 and IL-1 RAcP, and this is antagonized by IL-36Ra which also binds IL-1 Rrp2 (11, 12). The potency of IL-36 beta is increased by cleavage of its first four N-terminal amino acids (13).
References
- Garlanda, C. et al. (2013) Immunity 39:1003.
- Gresnigt, M.S. and F.L. van de Veerdonk (2013) Semin. Immunol. 25:458.
- Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
- Johnston, A. et al. (2011) J. Immunol. 186:2613.
- Wang, P. et al. (2005) Cytokine 29:245.
- Vigne, S. et al. (2012) Blood 120:3478.
- Magne, D. et al. (2005) Arthritis Res. Ther. 8:R80.
- Carrier, Y. et al. (2011) J. Invest. Dermatol. 131:2428.
- Foster, A.M. et al. (2014) J. Immunol. 192:6053.
- Vigne, S. et al. (2011) Blood 118:5813.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
- Towne, J.E. et al. (2011) J. Biol. Chem. 286:42594.
Long Name
Interleukin 36 beta/Interleukin 1 Family 8
Alternate Names
FIL1 eta, IL-1 eta, IL-1H2, IL1F8, IL36 beta, IL36B
Gene Symbol
IL36B
UniProt
Additional IL-36 beta/IL-1F8 Products
Product Documents for Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF
Product Specific Notices for Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF
For research use only
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