Recombinant Human IL-36 gamma/IL-1F9 Protein

R&D Systems, part of Bio-Techne | Catalog # 2320-IL

R&D Systems, part of Bio-Techne
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Carrier Free
2320-IL-025/CF
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With Carrier
2320-IL-025
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Key Product Details

Source

E. coli

Accession #

Q9NZH8

Conjugate

Unconjugated

Applications

Binding Activity

View all IL-36 gamma/IL-1F9 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-36 gamma/IL-1F9 protein
Met1-Asp169

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

18.7 kDa

Activity

Measured by its binding ability in a functional ELISA.
Immobilized recombinant human IL-36 gamma at 1 µg/mL (100 µL/well) can bind recombinant human IL-1 Rrp2 Fc Chimera (Catalog # 872-RP) with a linear range of 0.15 ‑ 5 µg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2320-IL
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2320-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 gamma/IL-1F9

Human interleukin 36 gamma (IL‑36 gamma; formerly known as IL‑1F9, IL‑1 epsilon (epsilon) and IL‑1H1) is a secreted member of the IL‑1 family of proteins (1‑5). It currently is one of at least four IL‑1 family members that have been renamed, the others being IL‑36 alpha (formerly IL‑1F6), IL‑36 beta (formerly IL‑1F8) IL‑37 (formerly IL‑1F7) and IL‑36ra (formerly IL‑1F5) (1). All family members show a 12 beta‑strand, beta‑trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (6). IL‑36 gamma is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation site(s) (3, 4, 7). The molecule is secreted via a nonclassical pathway and likely requires the presence of extracellular ATP (7, 8). Human to mouse, IL‑36 gamma shares 53% aa identity. Within the family, IL‑36 gamma shares 30% aa identity with IL‑1ra, and 23%, 33%, 57%, 35%, 45% and 32% aa identity with IL‑1 beta, IL‑36ra, IL‑36 alpha, IL‑37, IL‑36 beta and IL‑1F10, respectively. Cells reported to express IL‑36 gamma include Langerhans cells, keratinocytes, monocytes, bronchial epithelium plus Chief cells and Parietal cells of the stomach (6 ‑ 10). The receptor for IL‑36 alpha is reported to be a combination of IL‑1Rrp2 and IL‑1 RAcP (9). Recombinant IL‑136 gamma, along with IL‑36 alpha and IL‑36 beta, has been shown to act as an agonist by activating the pathway involving NF‑ kappaB and MAPK in an IL‑1Rrp2 dependent manner. This suggests that IL‑36 gamma may signal in similar fashion to IL‑1 and IL‑18 by having a binding receptor which, upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex. Activities attributed to IL‑36 gamma include a down‑regulation of betacellulin, an up‑regulation of MMP‑9 and ‑10, and the activation of both macrophages and fibroblasts, resulting in the release of multiple chemokines such as CXCL1, 2, 3 and 8, plus CCL2, 3 and 20 (9 ‑ 11).

References

  1. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  2. barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  3. Smith, D. E. et al. (2000) J. Biol. Chem. 275 :1169.
  4. Kumar, S. et al. (2000) J. Biol. Chem. 275 :10308.
  5. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  6. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  7. Debets, R. et al. (2001) J. Immunol. 167:1440.
  8. Johnston, A. et al. (2011) J. Immunol. 186:2613.
  9. Chustz, R.T. et al. (2010) Am. J. Respir. Cell Mol. Biol. [Epub ahead of print].
  10. Ramadas, R.A. et al. (2011) Am. J. Respir. Cell Mol. Biol. 44:134.
  11. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 36 gamma/Interleukin 1 Family 9

Alternate Names

IL-1 H1, IL-1rp2, IL1F9, IL36 gamma, IL36G

Entrez Gene IDs

56300 (Human); 215257 (Mouse); 499744 (Rat)

Gene Symbol

IL36G

UniProt

Q9NZH8

Additional IL-36 gamma/IL-1F9 Products

Product Documents for Recombinant Human IL-36 gamma/IL-1F9 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 2320-IL (with carrier)
Download SDS 2320-IL/CF (carrier free)

Product Specific Notices for Recombinant Human IL-36 gamma/IL-1F9 Protein

For research use only

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