Recombinant Human IL-39 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9990-IL

R&D Systems, part of Bio-Techne
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9990-IL-050
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Key Product Details

Source

HEK293

Accession #

Q14213, Q9NPF7

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-39 (IL-23p19/EBI3) Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-39 (IL-23p19/EBI3) protein
Human EBI3
(Arg21-Lys229, Gln95Cys)
Accession # Q14213		 GGGSGGGSGGGS
 Human IL-23p19
(Arg20-Pro189)
Accession # Q9NPF7		 IEGRMD Human IgG1
(Pro100-Lys330)

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg21 (EBI3)

Predicted Molecular Mass

69 kDa

SDS-PAGE

70-77 kDa, reducing conditions

Activity

Measured by its ability to induce IFN-gamma production by mouse splenocytes.
The ED50 for this effect is 50-250 ng/mL.

Scientific Data Images for Recombinant Human IL-39 Fc Chimera Protein, CF

Recombinant Human IL-39 Fc Chimera Protein Bioactivity

Recombinant Human IL-39 Fc Chimera Protein Bioactivity

Recombinant Human IL-39 Fc Chimera (Catalog # 9990-IL) induces IFN-gamma production in mouse splenocytes. The ED50 for this effect is 50-250 ng/mL.
Recombinant Human IL-39 Fc Chimera Protein SDS-PAGE

Recombinant Human IL-39 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human IL-39 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-77 kDa and 140-160 kDa, respectively.

Formulation, Preparation and Storage

9990-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IL-39 (IL-23p19/EBI3)

Interleukin 39 (IL-39) is a member of the IL-12 family of heterodimeric cytokines. IL-12 cytokines are composed of an alpha and beta subunit which, for IL-39 are the IL-23 p19 subunit and the EBI3 subunit, respectively (1-3). The IL-23 p19 subunit of IL-39 is synthesized as a 189 amino acid (aa) precursor protein with a 19 aa signal sequence and a 170 aa mature region. The EBI3 subunit of IL-39 is synthesized as a 229 aa precursor protein that contains a 20 aa signal sequence and a 209 aa mature region. Human and mouse IL-39 share 73% and 62% sequence homology in their IL-23 p19 and EBI3 subunits, respectively. Heterodimer (IL-39) is secreted by LPS-stimulated B cells and GL7(+) activated B cells of lupus-like mice (1, 4). IL-39 signals through IL-23 R/gp130 receptors and mediates inflammatory responses through activation of STAT1 and STAT3 in lupus-like mice (1, 2). IL‐39 induces and/or expands neutrophils in lupus‐prone mice, and IL‐39‐induced neutrophils increase the secretion of B cell activation factor (BAFF) (3). Thus, IL-39 is an important pro-inflammatory cytokine and play important roles in the pathophysiology of autoimmune diseases, including Systemic Lupus Erythematosus (SLE) (2, 4).

References

  1. Wang, X. et al. (2016) Eur J Immunol 46:1343.
  2. Hasegawa, H. et al. (2016) Front Immunol 7:479.
  3. Wang, X. et al. (2016) Clin Exp Immunol 186:144.
  4. Wang, X. et al. (2018) Mol Med Rep 17:1660.

Long Name

Interleukin 39 (IL-23p19/EBI3 Heterodimer)

Alternate Names

IL-23 p19/EBI3, IL-23p19/EBI3

Entrez Gene IDs

51561 (Human)

Gene Symbol

IL23A

UniProt

Q14213, Q9NPF7

Additional IL-39 (IL-23p19/EBI3) Products

Product Documents for Recombinant Human IL-39 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-39 Fc Chimera Protein, CF

For research use only

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