Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI7700

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P24394.1

Structure / Form

Disulfide-linked homodimer, Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Binding Activity, Bioactivity

View all IL-4R alpha Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-4R alpha protein
Human IL-4R alpha
(Gly24-His232)
Accession # P24394.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

52 kDa

SDS-PAGE

73-82 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 5-30 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL‑4 (Catalog # 204-IL).

Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human IL-4R alpha Fc Chimera Avi-tag (Catalog # AVI7700) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004), the concentration of Recombinant Human IL-4 (Catalog # 204-IL) that produces 50% of the optimal binding response is approximately 3-20 ng/mL.

Scientific Data Images for Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF

Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein Binding Activity

Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein Binding Activity

When Biotinylated Recombinant Human IL-4R alpha Fc Chimera Avi-tag (Catalog # AVI7700) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004), the concentration of Recombinant Human IL-4 (Catalog # 204-IL) that produces 50% of the optimal binding response is approximately 3-20 ng/mL.
Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein SDS-PAGE

Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human IL-4R alpha Fc Chimera Avi-tag (Catalog # AVI7700) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-82 kDa and 145 -165 kDa, respectively.

Formulation, Preparation and Storage

AVI7700
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4R alpha

Interleukin 4 Receptor alpha (IL-4R alpha), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1-5). Mature human IL-4R alpha consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6, 7). Within the ECD, human IL-4R alpha shares 51% aa sequence identity with mouse and rat IL-4R alpha. Soluble forms of IL-4R alpha, generated by alternate splicing or proteolysis, retain ligand binding properties and inhibit IL-4 bioactivity (8-11). IL-4R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (6). It can associate with the common gamma chain ( gammac) to form the IL-4 responsive type I receptor in which gammac increases the affinity for IL-4 and enables signaling (12, 13). It can alternatively associate with IL-13R alpha1 to form the type II receptor which is responsive to both IL-4 and IL-13 (14, 15). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize gammac (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (16, 17).

References

  1. Wills-Karp, M. and F.D. Finkelman (2008) Sci. Signal. 1:pe55.
  2. Gordon, S. and F.O. Martinez (2010) Immunity 32:593.
  3. Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384.
  4. Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415.
  5. Lee, Y.W. et al. (2010) Biomol. Ther. 18:135.
  6. Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861.
  7. Galizzi, J.P. et al. (1990) Int. Immunol. 2:669.
  8. Kruse, S. et al. (1999) Int. Immunol. 11:1965.
  9. Blum, H. et al. (1996) J. Immunol. 157:1846.
  10. Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23.
  11. Mosley, B. et al. (1989) Cell 59:335.
  12. Kondo, M. et al. (1993) Science 262:1874.
  13. Russell, S.M. et al. (1993) Science 262:1880.
  14. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497.
  15. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  16. Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25.
  17. Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.

Long Name

Interleukin 4 Receptor alpha

Alternate Names

CD124, IL-4 R alpha, IL-4Ra, IL4R, IL4R alpha

Entrez Gene IDs

3566 (Human); 16190 (Mouse); 102133649 (Cynomolgus Monkey)

Gene Symbol

IL4R

UniProt

P24394.1

Additional IL-4R alpha Products

Product Documents for Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF

For research use only

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