Recombinant Human IL-9R (NS0-expressed) Protein
R&D Systems, part of Bio-Techne | Catalog # 290-RNS
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human IL-9 R protein
Val40-Pro270, with a C-terminal 10-His tag
Val40-Pro270, with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val40
Predicted Molecular Mass
27.4 kDa
SDS-PAGE
38 kDa, reducing conditions
Activity
Measured by its ability to inhibit the IL-9-dependent proliferation of MO7e human megakaryocytic leukemic cells.
The ED50 for this effect is 1-4 µg/mL in the presence of 2 ng/mL of recombinant human IL-9.
The ED50 for this effect is 1-4 µg/mL in the presence of 2 ng/mL of recombinant human IL-9.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 290-RNS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 290-RNS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-9R
The specific high affinity functional IL-9 receptor complex consists of a ligand-specific IL-9 R( alpha) subunit and the common gamma chain that is shared with the receptors for IL-2, IL-4, IL-7 and IL-15. Both the IL-9 R subunit and the common gamma chain are members of the hematopoietin receptor superfamily. The cDNAs encoding mouse and human IL-9 receptors have been isolated. The deduced mouse and human transmembrane proteins, sharing 53% amino acid sequence identity, contain 468 and 533 amino acid residues, respectively. As a result of alternative splicing, cDNA clones encoding isoforms of IL-9 R, including a putative soluble form, have also been identified. Cells known to express IL-9 R include T cells, neutrophils, mast cells and macrophages.
References
- Renauld, J. et al. (1992) Proc. Natl. Acad. Sci. USA, 89:5690.
- Kimura, Y. et al. (1995) Int. Immunol. 7:115.
Long Name
Interleukin 9 Receptor
Alternate Names
CD129, IL-9 R, IL9R
Gene Symbol
IL9R
UniProt
Additional IL-9R Products
Product Documents for Recombinant Human IL-9R (NS0-expressed) Protein
Product Specific Notices for Recombinant Human IL-9R (NS0-expressed) Protein
For research use only
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