Recombinant Human ILDR2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9991-IR

R&D Systems, part of Bio-Techne
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9991-IR-050
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Key Product Details

Source

CHO

Accession #

Q71H61

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ILDR2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ILDR2 protein
Human ILDR2
(Leu21 - Glu186)
Accession # Q71H61		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu21

Predicted Molecular Mass

45 kDa

SDS-PAGE

47-52 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 1-6 μg/mL.

Scientific Data Images for Recombinant Human ILDR2 Fc Chimera Protein, CF

Recombinant Human ILDR2 Fc Chimera Protein Bioactivity

Recombinant Human ILDR2 Fc Chimera Protein Bioactivity

Recombinant Human ILDR2 Fc Chimera (Catalog # 9991-IL) inhibits IFNg secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-6 μg/mL.

Formulation, Preparation and Storage

9991-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: ILDR2

ILDR2 (Immunoglobulin-like domain-containing receptor 2) is a member of the B7-like family of proteins that regulate T cell activity (1). ILDR2 is also a known endoplasmic reticulum molecule that regulates lipid homeostasis (2, 3). It contains a signal peptide, an Ig-like V-type domain, a stalk region, a transmembrane domain and a CCP-rich domain (1, 4). The human ILDR2 lumenal domain shares a 99% and 98% homology with the mouse and rat respectively. The human gene encoding ILDR2 is located in a region on Chr1q23–25 that has been associated with type 2 diabetes (5). ILDR2 and its two paralogs, ILDR1 and lipolysis-stimulated receptor (LSR; also named ILDR3), are members of angulin family proteins (angulin-1/LSR, angulin-2/ILDR1, and angulin-3/ILDR2), and they are identified as protein components of tricellular tight junctions (tTJs), which are required for recruitment of tricellulin to tTJs (6). ILDR2 plays critical roles in hepatic clearance of lipoproteins and in lipid homeostasis (3). ILDR2 regulates human dendritic cells (DC2 cells, a subpopulation of polarized DCs that promotes Th2 differentiation) (7). Recent publications reported that ILDR2 displayed negative regulatory functions on human and mouse T cells in various experimental systems. Fusion protein of ILDR2 lumenal domain with an Fc fragment, displays therapeutic effects in collagen-induced arthritis (CIA), a mouse model of rheumatoid arthritis (RA). ILDR2 represents a novel B7-like ligand that exerts negative immune modulation via interaction with a putative counterpart receptor expressed on activated T cells (1, 4).

References

  1. Hecht, I. et al. (2018) J. Immunol. 200:2025.
  2. Watanabe, K. et al. (2013) PLoS One. 8:e67234.
  3. Watanabe, K. et al. (2016) Biochem. Biophys. Res. Commun. 477:712.
  4. Podojil, J. R. et al. (2018) J. Immunol. 200:2013.
  5. Dokmanovic-Chouinard, M. et al. (2008) PLoS Genet. 4:e1000137.
  6. Higashi, T. et al. (2013) J. Cell Sci. 126:966.
  7. Gueguen, C. et al. (2016) J. Allergy Clin. Immunol. 137:545.

Long Name

Immunoglobulin Like Domain Containing Receptor 2

Alternate Names

Angulin-3, C1orf32, Dbsm1, DJ782G3.1, LISCH-Like

Entrez Gene IDs

387597 (Human); 100039795 (Mouse); 685277 (Rat)

Gene Symbol

ILDR2

UniProt

Q71H61

Additional ILDR2 Products

Product Documents for Recombinant Human ILDR2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human ILDR2 Fc Chimera Protein, CF

For research use only

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