Recombinant Human Inhibin A Protein

R&D Systems, part of Bio-Techne | Catalog # 8506-AB

R&D Systems, part of Bio-Techne
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Carrier Free
8506-AB-010/CF
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With Carrier
8506-AB-010
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Key Product Details

Source

CHO

Accession #

P05111 (alpha chain) & P08476 (beta chain)

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Inhibin A Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Inhibin A protein
Ser233-Ile366 (alpha chain) & Gly311-Ser426 (beta chain)

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser233 (alpha chain) & Gly311 (beta chain)

Predicted Molecular Mass

15 kDa (alpha chain) & 13 kDa (beta chain)

SDS-PAGE

12-25 kDa, reducing conditions

Activity

Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells.
The ED50 for this effect is 3-18 ng/mL.

Reviewed Applications

Read 1 review rated 4 using 8506-AB in the following applications:

Scientific Data Images for Recombinant Human Inhibin A Protein

Recombinant Human Inhibin A Protein Bioactivity

Recombinant Human Inhibin A Protein Bioactivity

Recombinant Human Inhibin A (Cat# 8506-AB) neutralizes Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 3-18 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8506-AB
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8506-AB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Inhibin A

Inhibin A is a member of the TGF-beta superfamily of proteins (1). Mature Inhibin A is a disulfide-linked dimer composed of alpha and beta subunits (1). Inhibin A shares a common alpha subunit with the closely related protein Inhibin B but has a unique beta subunit ( betaA) (2, 3). The mature alpha subunit of human Inhibin A has a predicted molecular weight of 18 kDa and shares 80% amino acid (aa) sequence identity with the mouse and rat orthologs (2, 4). The mature beta subunit of this human protein has a predicted molecular weight of 13 kDa and shares 100% aa sequence identity with the mouse and rat orthologs. Inhibin binds and antagonizes ActRIIA and ActRIIB in complex with the TGF-beta RIII and/or IGSF1 co-receptors and subsequently acts to suppress Activin-induced Follicle Stimulating Hormone (FSH) secretion (1, 5-7). Inhibins are produced by gonadal cells in both males and females (8). They are thought to be involved in the regulation of gametogenesis, and embryonic and fetal development (8, 9). Elevated concentrations of Inhibins are associated with pregnancy, preeclampsia, and ovarian cancer, and Inhibin A levels are typically measured during prenatal screening for Down’s syndrome (10-14).

References

  1. Phillips, D.J. and T.K. Woodruff (2004) Growth Factors 22:13.
  2. Mason, A.J. et al. (1986) Biochem. Biophys. Res. Commun. 135:957.
  3. Ying, S.Y. (1987) Proc. Soc. Exp. Biol. Med. 186:253.
  4. Mayo, K.E. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5849.
  5. Lewis, K.A. et al. (2000) Nature 404:411.
  6. Martens, J.W. et al. (1997) Endocrinology 138:2928.
  7. Chapman, S.C. et al. (2002) Mol. Cell. Endocrinol. 196:79.
  8. de Kretser, D.M. and D.M. Robertson (1989) Biol. Reprod. 40:33.
  9. Knight, P.G. et al. (2012) Mol. Cell. Endocrinol. 359:53.
  10. Walentowicz, P. et al. (2014) PLoS One 9:e90575.
  11. Kondi-Pafiti, A. et al. (2013) Clin. Exp. Obstet. Gynecol. 40:109.
  12. Kuijper, E.A. et al. (2013) Reprod. Biomed. Online 27:33.
  13. Carty, D.M. et al. (2008) Trends Cardiovasc. Med. 18:186.
  14. Wald, N.J. et al. (1996) Prenat. Diagn. 16:143.

Alternate Names

INHA, Inhibin A

Entrez Gene IDs

3623 (Human); 16322 (Mouse)

Gene Symbol

INHA

UniProt

P05111 (alpha chain) & P08476 (beta chain)

Additional Inhibin A Products

Product Documents for Recombinant Human Inhibin A Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8506-AB (with carrier)
Download SDS 8506-AB/CF (carrier free)

Product Specific Notices for Recombinant Human Inhibin A Protein

For research use only

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