Recombinant Human Integrin alpha 2 beta 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5698-A2

R&D Systems, part of Bio-Techne
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5698-A2-050
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Key Product Details

Source

CHO

Accession #

NP_002194 (Integrin alpha2) & P05556 (Integrin beta1)

Conjugate

Unconjugated

Applications

Binding Activity

View all Integrin alpha 2 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 2 beta 1 protein
Human Integrin alpha2
(Tyr30-Thr1132)
Accession # NP_002194		 HPGGGSGGGS Human c-Jun
(Arg276-His315)
Human Integrin beta1
(Gln21-Asp728)
Accession # P05556		 GGGSGGGS Human c-Fos
(Leu161-Ala199)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr30 ( alpha2) & No results obtained, Gln21 predicted ( beta1)

Predicted Molecular Mass

126 kDa ( alpha2) & 83.3 kDa ( beta1)

SDS-PAGE

140-150 kDa ( alpha2) & 120-130 kDa ( beta1), reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When bovine collagen-II is coated at 10 μg/mL, Recombinant Human Integrin alpha2 beta1 binds with an apparent Kd <1nM.

Formulation, Preparation and Storage

5698-A2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2 beta 1

Integrin alpha2 beta1 is one of twelve integrin family adhesion receptors that share the beta1 subunit (1-3). It is the non-covalent heterodimer of 160 kDa alpha2 (CD49b) and 130 kDa beta1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha2 extracellular domain (ECD) shares 83-89% aa sequence identity with mouse, rat, canine, bovine and equine alpha2, while the 708 aa human beta1 ECD shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta1. The I domain-containing beta1 integrins ( alpha1 beta1, alpha2 beta1, alpha10 beta1 and alpha11 beta1) all bind collagens, with alpha2 beta1 preferring collagens I-III (4, 5). Platelet alpha2 beta1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha2 beta1 ligands include laminin, decorin, E‑cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha1 beta1 (8-10). In addition to expression on selected hematopoietic cells, alpha2 beta1 is present on a wide variety of non‑hematopoietic cells (4). Mice deficient in the alpha2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha2 beta1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
  4. Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
  5. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  6. Sarratt, K.L. et al. (2005) Blood 106:1268.
  7. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
  8. Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
  9. McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
  10. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
  11. Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
  12. Edelson, B.T. et al. 2006) Blood 107:143.

Alternate Names

alpha 2 integrin, alpha 2 subunit of VLA-2 receptor, BR, CD49 antigen-like family member B, CD49b, collagen receptor, glycoprotein Ia, GPIa, HPA-5, human platelet alloantigen 5, human platelet alloantigen system 5, integrin alpha-2, integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor), platelet antigen Br, platelet glycoprotein GPIa, platelet membrane glycoprotein Ia, very late activation protein 2 receptor, alpha-2 subunit, very late activation receptor alpha-2 subunit, very late activation receptor subunit alpha-2, VLA-2, VLAA2

Entrez Gene IDs

3673 (Human); 16401 (Mouse)

Gene Symbol

ITGA2

UniProt

NP_002194 (Integrin alpha2) & P05556 (Integrin beta1)

Additional Integrin alpha 2 beta 1 Products

Product Documents for Recombinant Human Integrin alpha 2 beta 1 Protein, CF

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Product Specific Notices for Recombinant Human Integrin alpha 2 beta 1 Protein, CF

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