Recombinant Human Kallikrein 1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2337-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Kallikrein 1 protein
Ile25-Ser262, with an N-terminal human KLK-5 propeptide (Val23-Arg66) and a C-terminal 10-His tag
Ile25-Ser262, with an N-terminal human KLK-5 propeptide (Val23-Arg66) and a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val23 (KLK-5 propeptide, Accession # Q9Y337)
Predicted Molecular Mass
32 kDa
SDS-PAGE
41 kDa, 45 kDa, 49 kDa and 56 kDa, reducing conditions
Activity
Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).
The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2337-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Kallikrein 1
Kallikrein 1 (KLK1), also known as tissue kallikrein, is a member of human tissue kallikrein family (1). The best known physiological function of KLK1 is the cleavage of kininogen to release the vasoactive kinin peptide (bradykinin or lysyl-bradykinin), which regulates vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation (2). In addition, KLK1 may play a role in angiogensis and tumorigenesis (2). Human KLK1 precursor contains a signal peptide (residues1 to 18), a short pro peptide (residues 19 to 24) and a mature chain (residues 25 to 262). The purified rhKLK1 contains the mature chain and the propeptide from human KLK5. After being activated by thermolysin, rhKLK1 is active against a fluorogenic peptide substrate described above.
References
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
- Chao, J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1577 - 1580.
Alternate Names
KLK1
Gene Symbol
KLK1
UniProt
Additional Kallikrein 1 Products
Product Documents for Recombinant Human Kallikrein 1 Protein, CF
Product Specific Notices for Recombinant Human Kallikrein 1 Protein, CF
For research use only
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