Recombinant Human Kallikrein 3/PSA Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1344-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Kallikrein 3/PSA protein
Ala18-Pro261, with a C-terminal 10-His tag
Ala18-Pro261, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala18
Predicted Molecular Mass
28 kDa
SDS-PAGE
36 kDa, reducing conditions
Activity
Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA).
The specific activity is >70 pmol/min/µg, as measured under the described conditions.
The specific activity is >70 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 2 reviews rated 5 using 1344-SE in the following applications:
Scientific Data Images for Recombinant Human Kallikrein 3/PSA Protein, CF
Recombinant Human Kallikrein 3/PSA Protein Enzyme Activity
Recombinant Human Kallikrein 3 (Catalog # 1344-SE) is measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA).Formulation, Preparation and Storage
1344-SE
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2. |
| Reconstitution | Reconstitute at 200 μg/mL in 50 mM Tris, 10 mM CaCl2,150 mM NaCl and 0.05% (w/v) Brij-35, pH 7.5. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Kallikrein 3/PSA
Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family (1). PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5 - 2 g/L) (2). A small portion of PSA “leaks” into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue (3). PSA has become a well established tumor marker that aids the diagnosis, staging, and follow up of prostate cancer.
The deduced amino acid sequence of human PSA consists of a signal peptide, a short pro region and a mature/active enzyme. The pro-enzyme is activated, possibly by active Kallikreins 2, 4 or 15 in vivo (4). rhPSA is activated by thermolysin, a zinc protease. The active PSA cleaves several tyrosyl peptide bonds in semenogelins I and II, which are the major gel-forming proteins produced by the seminal vesicles (5). Several inhibitors including serpin A3/ alpha1-antichymotrypsin (ACT) and alpha2‑macroglobulin are known to form complexes with PSA.
References
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
- Ward, A.M. et al. (2001) Ann. Clin. Biochem. 38:633.
- Jain, S. et al. (2002) Postgrad. Med. J. 78:646.
- LiLja H. (2003) Urology 62:27.
- Takayama, T.K. et al. (1997) J. Biol. Chem. 272:21582.
Alternate Names
KLK3, PSA
Entrez Gene IDs
354 (Human)
Gene Symbol
KLK3
UniProt
Additional Kallikrein 3/PSA Products
Product Documents for Recombinant Human Kallikrein 3/PSA Protein, CF
Product Specific Notices for Recombinant Human Kallikrein 3/PSA Protein, CF
For research use only
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