Recombinant Human KGF/FGF-7 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11340-KG

R&D Systems, part of Bio-Techne
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11340-KG-010
11340-KG-01M
11340-KG-050
11340-KG-100
11340-KG-500
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Key Product Details

Source

HEK293

Accession #

P21781.1

Conjugate

Unconjugated

Applications

Bioactivity

View all KGF/FGF-7 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human KGF/FGF-7 protein
Cys32-Thr194

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys32

Predicted Molecular Mass

19 kDa

SDS-PAGE

22-28 kDa, under reducing conditions.

Activity

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 25.0-250 ng/mL.

Scientific Data Images for Recombinant Human KGF/FGF-7 Protein, CF

Recombinant Human KGF/FGF‑7 Protein Bioactivity.

Recombinant Human KGF/FGF-7 Protein (Catalog # 11340-KG) stimulates cell proliferation of MCF-7 human breast cancer cells, The ED50 for this effect is 25.0-250 ng/mL.

Recombinant Human KGF/FGF‑7 Protein SDS-PAGE.

2 μg/lane of Recombinant Human KGF/FGF‑7 Protein (Catalog # 11340-KG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 22-28 kDa.

Formulation, Preparation and Storage

11340-KG
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with Trehalose.
Reconstitution Reconstitute 10 μg size at 100 μg/mL and all the other sizes at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KGF/FGF-7

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1-4,13). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

References

  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.
  13. Vadija R. et al. (2016) J Chem Biol. 9:69.

Long Name

Keratinocyte Growth Factor

Alternate Names

FGF-7, FGF7, HBGF-7, HBGF7

Entrez Gene IDs

2252 (Human); 403915 (Canine)

Gene Symbol

FGF7

UniProt

P21781.1

Additional KGF/FGF-7 Products

Product Documents for Recombinant Human KGF/FGF-7 Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human KGF/FGF-7 Protein, CF

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