Recombinant Human KGF/FGF-7 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11537-KG

CHO expressed
R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

P21781.1

Conjugate

Unconjugated

Applications

Bioactivity

View all KGF/FGF-7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human KGF/FGF-7 protein
Ser55-Thr194

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser55

Predicted Molecular Mass

16 kDa

SDS-PAGE

15-18 kDa, under reducing conditions

Activity

Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED50 for this effect is 4.00-40.0 ng/mL.

Scientific Data Images for Recombinant Human KGF/FGF-7 Protein, CF

Recombinant Human KGF/FGF‑7 Protein Bioactivity.

Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED50 for this effect is 4.00-40.0 ng/mL.

Recombinant Human KGF/FGF‑7 Protein SDS-PAGE.

2 μg/lane of Recombinant Human KGF/FGF‑7 Protein (Catalog # 11537-KG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 15-18 kDa, under reducing conditions.

Formulation, Preparation and Storage

11537-KG
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with Trehalose.
Reconstitution Reconstitute 10 μg size at 100 μg/mL and the other sizes at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KGF/FGF-7

Fibroblast growth factor 7 (FGF7), also known as keratinocyte growth factor (KGF), is a member of the FGF family of secreted glycoproteins which are involved in key roles from development, morphogenesis, and angiogenesis to wound healing and tumorigenesis (1-4). FGF7, along with FGF3, FGF10, and FGF22, form a subfamily of the larger FGF family whose expression is restricted to cells of mesenchymal origin and function as paracrine growth factors for nearby epithelial cells (1). The FGF family is characterized by a core heparin-binding FGF domain of approximately 120 amino acids (aa) that exhibits a conserved beta -trefoil structure (2). Mature human FGF7 shares 96% and 92% aa sequence identity with mouse and rat FGF7, respectively. FGF7 signals only through the IIIb splice form of the receptor FGFR2, whose expression is restricted primarily to epithelial cells (5). FGF7 is noticeably upregulated in response to damage to skin or internal structures and is proposed to help speed-up wound healing (6). Deletion of FGF7 affects kidney development, producing abnormally small ureteric buds and fewer nephrons (7). FGF7 has been proposed as a potential cancer therapeutic by targeting the intermolecular interaction site between FGF7 and FGFR2b (3). Additionally, the use of FGF7 is being explored as novel treatment options for cartilage diseases, such as Osteoarthritis and Rheumatoid arthritis (8). An N-terminally truncated version of FGF7 with improved thermal stability, named Palifermin (brand name Kepivance), is approved for treatment for reducing and/or healing damages caused by cancer chemotherapy and radiotherapy, such as severe oral mucositis (OM) (9,10).

References

  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Itoh, N. and D.M. Ornitz (2008) Dev. Dyn. 237:18.
  3. Vadija, R. et al. (2016) J. Chem. Biol. 9:69.
  4. Itoh, N. and D.M. Ornitz (2004). Trends Genet. 20:563.
  5. Steiling, H. and S. Werner (2003) Curr. Opin. Biotechnol. 14:533.
  6. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  7. Qiao, J. et al. (1999) Development 126:547.
  8. Zhang, X. et al. (2023) Life Sci. 326:121804.
  9. Hsu, E. et al. (2006) PEDS 19:147.
  10. Sadeghi, S. et al. (2021)  Int. J. Biol. Macromol. 191:1175.

Long Name

Keratinocyte Growth Factor

Alternate Names

FGF-7, FGF7, HBGF-7, HBGF7

Entrez Gene IDs

2252 (Human); 403915 (Canine)

Gene Symbol

FGF7

UniProt

P21781.1

Additional KGF/FGF-7 Products

Product Documents for Recombinant Human KGF/FGF-7 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human KGF/FGF-7 Protein, CF

For research use only

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