Recombinant Human KIR2DL3/CD158b2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2014-KR

R&D Systems, part of Bio-Techne
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2014-KR-050
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Key Product Details

Source

NS0

Accession #

P43628

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all KIR2DL3/CD158b2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human KIR2DL3/CD158b2 protein
Human KIR2DL3
(His22-His245)
Accession # P43628		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His22

Predicted Molecular Mass

51.2 kDa (monomer)

SDS-PAGE

66-75 kDa, reducing conditions

Activity

Measured by its ability to bind HEK293T human embryonic kidney cells in a flow cytometry assay.
When 10 μg of Recombinant Human KIR2DL3/CD158b2 Fc Chimera is added to 5 x 105 HEK293T cells, >20% of the cells will bind to the protein.

Reviewed Applications

Read 2 reviews rated 4 using 2014-KR in the following applications:

Formulation, Preparation and Storage

2014-KR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR2DL3/CD158b2

KIR2DL3 (2DL3, formerly NKAT2, designated CD158b2) is a 341 amino acid (aa) type I transmembrane glycoprotein that belongs to the human killer cell Ig‑like receptor (KIR) family of molecules (1, 2). KIRs are expressed on human CD56dim NK cells and T cell subsets, and regulate effector functions in the innate immune system (1-3). KIRs are named for the number of Ig‑like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails (1‑3). Individuals will express varying subsets of inhibiting and activating KIRs with varying polymorphisms (1, 4). Like other inhibiting KIRs, KIR2DL3 has two ITIM domains within its long tail that block activating receptor clustering (2). Within the ECD, KIR2DL3 shares very high aa sequence identity (98%) with KIR2DL2. The two segregate as alleles of the same gene; both recognize Asn80‑containing HLA‑C1 and, more weakly, Lys80‑containing C2 allotypes (1, 5). Extracellular aa identity is also high for KIR2DL1 (93%). The three molecules together recognize and inhibit NK cytotoxicity against cells expressing any HLA‑C allotype, allowing for self‑recognition (1‑3). Compared with KIR2DL2, KIR2DL3 shows lower avidity for HLA‑C1 ligands; when compared to KIR2DL1, KIR2DL3 has lower avidity but broader specificity for HLA-C1 ligands (1, 5, 6). Configurations of inhibiting and activating KIR can alter an individual’s susceptibility to viral and autoimmune diseases and leukemia (1‑3). For example, rheumatoid arthritis patients that are positive for KIR2DL3 and negative for KIR2DS3 have an earlier disease diagnosis (7).

References

  1. Purdy, A.K. and Campbell, K.S. (2009) Cancer Biol. Ther. 8:13.
  2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  3. Kulkarni, S. et al. (2008) Semin. Immunol. 20:343.
  4. Middleton, D. and F. Gonzelez (2009) Immunology 129:8.
  5. Moesta, A.K. et al. (2008) J. Immunol. 180:3969.
  6. Hilton, H.G. et al. (2012) J. Immunol. 189:1418.
  7. Majorczyk, E. et al. (2007) Genes Immun. 8:678.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 3

Alternate Names

CD158b2, NKAT2

Entrez Gene IDs

3804 (Human)

Gene Symbol

KIR2DL3

UniProt

P43628

Additional KIR2DL3/CD158b2 Products

Product Documents for Recombinant Human KIR2DL3/CD158b2 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human KIR2DL3/CD158b2 Fc Chimera Protein, CF

For research use only

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