Recombinant Human KIR3DL3/CD158z Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10104-KR

R&D Systems, part of Bio-Techne
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10104-KR-050
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Key Product Details

Source

HEK293

Accession #

NP_703144.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all KIR3DL3/CD158z Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human KIR3DL3/CD158z protein
Human KIR3DL3/CD158z
(Gln26-Leu322)
Accession # NP_703144.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln26; blocked

Predicted Molecular Mass

59 kDa

SDS-PAGE

62-71 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human B7-H7/HHLA2 His-tag Protein, CF (Catalog # 10475-B7) is immobilized at 2 μg/mL (100 µL/well), the concentration of Recombinant Human KIR3DL3/CD158z Fc Chimera (Catalog # 10104-KR) that produces 50% of the optimal binding response is found to be approximately 45.0-270 ng/mL.

Scientific Data Images for Recombinant Human KIR3DL3/CD158z Fc Chimera Protein, CF

Recombinant Human KIR3DL3/CD158z Fc Chimera Protein Binding Activity.

When Recombinant Human B7-H7/HHLA2 His-tag (10475-B7) is immobilized at 2 μg/mL (100 µL/well), the concentration of Recombinant Human KIR3DL3/CD158z Fc Chimera (Catalog # 10104-KR) that produces 50% of the optimal binding response is found to be approximately 45.0-270 ng/mL

Recombinant Human KIR3DL3/CD158z Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human KIR3DL3/CD158z Fc Chimera (Catalog # 10104-KR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-71 kDa and 120-140 kDa.

Formulation, Preparation and Storage

10104-KR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR3DL3/CD158z

KIR3DL3 (also known as CD158z, KIR3DL7, KIR44, or KIRC1) is a type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1-4). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. Human KIR3DL3 cDNA encodes a 410 amino acid (aa) polypeptide precursor with a 25 aa signal peptide, a 297 aa extracellular domain (ECD) with 3 Ig-like domains (3D), a 21 aa transmembrane domain, and a 67 aa cytoplasmic domain (long). Within ECD human KIR3DL3 shares 47% and 44% aa sequence identity with mouse and rat KIR3DL3, respectively. KIR3DL3 is ubiquitously present in every individual across diverse populations, however little is known about specific functions (5). The limited knowledge of KIR3DL3 expression does suggest involvement in reproduction, likely during placentation (4). KIR3DL3 likely encodes an NK cell inhibitory receptor (6). Recent studies have shown that KIR3DL3 binding and function require both receptor aggregation and inhibitory signal attenuation (7).

References

  1. Colonna, M. and J. Samaridis (1995) Science 268:405.
  2. Lanier, L.L. (2005) Annu. Rev. Immunol. 23:225.
  3. Uhrberg, M. et al. (1997) Immunity 7:753.
  4. Trundley, A.E. et al. (2006) Immunogenetics 57:904.
  5. Hollenbach, J.A. et al. (2012) Immunogenetics 64:719.
  6. Torkar, M. et al. (1998) Eur. J. Immunol. 28:3959.
  7. Leaton, L.A. et al. (2019) Front. Immunol. 10:24.

Long Name

Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 3

Alternate Names

CD158z, KIR3DL7, KIR44, KIRC1

Entrez Gene IDs

115653 (Human)

Gene Symbol

KIR3DL3

UniProt

NP_703144.2

Additional KIR3DL3/CD158z Products

Product Documents for Recombinant Human KIR3DL3/CD158z Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human KIR3DL3/CD158z Fc Chimera Protein, CF

For research use only

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