Recombinant Human Kirrel1/NEPH1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10165-K1

Analyzed by SEC-MALS
R&D Systems, part of Bio-Techne
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10165-K1-050
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Key Product Details

Source

HEK293

Accession #

Q96J84

Conjugate

Unconjugated

Applications

Bioactivity

View all Kirrel1/NEPH1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Kirrel1/NEPH1 protein
Gln17-Leu493, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr21

Predicted Molecular Mass

53 kDa

SDS-PAGE

65-75 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Kirrel1/NEPH1 His-tag (Catalog # 10165-K1) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Nephrin (Catalog # 9399-NN) that produces 50% of the optimal binding response is 1-6 μg/mL.

Scientific Data Images for Recombinant Human Kirrel1/NEPH1 His-tag Protein, CF

Recombinant Human Kirrel1/NEPH1 His-tag Protein SEC-MALS.

Recombinant Human KIRREL-1/C-His Protein (Catalog # 10165-K1) has a molecular weight (MW) of 100.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human Kirrel1/NEPH1 His-tag Protein SDS-PAGE

Recombinant Human Kirrel1/NEPH1 His-tag Protein SDS-PAGE

2 µg/lane of Recombinant Human Kirrel 1/NEPH1 His-tag (Catalog # 10165-K1) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing major bands at 65-75 kDa and 50-60 kDa, respectively.

Formulation, Preparation and Storage

10165-K1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kirrel1/NEPH1

Kirrel1, also called NEPH1, is a 90-110 kDa type I transmembrane glycoprotein that belongs to the NEPH family of the immunoglobulin superfamily (1-4). The 757 amino acid (aa) human Kirrel1 contains a 16 aa signal sequence, a 483 aa extracellular domain (ECD) with five C2-type Ig-like domains, a 21 aa transmembrane sequence and a 237 aa cytoplasmic domain. The ECD also contains a site for FGF/FGF R interaction, and an RGD site that may allow integrin-mediated cell attachment. Five IgG-like repeats characterize the extracellular domain (5). The interaction of these five IgG-like motifs with the eight IgG-like motifs in Nephrin form a zipper-like meshwork around the glomerular capillaries in podocytes (5, 6). This interaction is what forms the structural basis of the slit diaphragm regulating macromolecule movement from the blood (5). Human Kirrel1 shares 98% aa identity with mouse and rat homologs, respectively, within the ECD. Kirrel1 expression has been mainly studied in the kidney glomerular slit diaphragm, but its expression with nephrin or other family members has also been reported in central nervous system neurons, pancreas and placenta (3, 4, 7-9). Kirrel1 forms cis hetero-oligomers with Nephrin, which brings together signaling molecules that direct actin polymerization (3, 4, 10). This interaction is essential for barrier function in the slit diaphragm, and mice deleted for Kirrel1 die perinatally due to proteinuria and failure to thrive (2, 3).

References

  1. Sellin, L. et al. (2003) FASEB J. 17:115.
  2. Donoviel, D.B. et al. (2001) Mol. Cell. Biol. 21:4829.
  3. Liu, G. et al. (2003) J. Clin. Invest. 112:209.
  4. Barletta, G-M. et al. (2003) J. Biol. Chem. 278:19266.
  5. Martin, E. and Jones, N. (2018) Front Endocrinal. 9:302.
  6. Grahammer, F. et al. (2016) JCI insight. 1: pii 86177.
  7. Gerke, P. et al. (2006) J. Comp. Neurol. 498:466.
  8. Rinta-Valkama, J. et al. (2007) Mol. Cell. Biochem. 294:117.
  9. Beall, M.H. et al. (2005) J. Soc. Gynecol. Investig. 12:298.
  10. Garg, P. et al. (2007) Mol. Cell. Biol. 27:8698.

Long Name

Kin of Irregular Chiasm-like 1

Alternate Names

KIRREL, NEPH1

Entrez Gene IDs

55243 (Human); 170643 (Mouse); 310695 (Rat)

Gene Symbol

KIRREL1

UniProt

Q96J84

Additional Kirrel1/NEPH1 Products

Product Documents for Recombinant Human Kirrel1/NEPH1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human Kirrel1/NEPH1 His-tag Protein, CF

For research use only

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