Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11169-LS

R&D Systems, part of Bio-Techne
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11169-LS-100
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Key Product Details

Source

CHO

Accession #

AAH20758.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all L-Selectin/CD62L Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human L-Selectin/CD62L protein
Human L-Selectin
(Trp52-Leu332)
Accession # AAH20758.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp52

Predicted Molecular Mass

58 kDa

SDS-PAGE

85-100 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells.
The ED50 for this effect is 0.350-3.50 μg/mL.

Scientific Data Images for Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

Recombinant Human L‑Selectin/CD62L Fc Chimera Protein Bioactivity.

Recombinant Human L‑Selectin/CD62L Fc Chimera Protein (Catalog # 11169-LS) supports the adhesion LS180 human colorectal adenocarcinoma cells. The ED50 for this effect is 0.350-3.50 μg/mL.

Recombinant Human L‑Selectin/CD62L Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human L‑Selectin/CD62L Fc Chimera Protein (Catalog # 11169-LS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-100 kDa and 170-200 kDa, respectively.

Formulation, Preparation and Storage

11169-LS
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: L-Selectin/CD62L

L-Selectin, also known as Leukocyte adhesion molecule 1 (LAM-1) and CD62L, is a type-1 cell surface glycoprotein and cell adhesion molecule of the Selectin family (1). In humans, there are 3 Selectins, P, E, and L, and they are Ca2+ dependent lectins that help mediate the initial adhesive step during inflammation and immune surveillance (2). Mature L-Selectin consists of an extracellular domain (ECD) with a C-type lectin domain and an epidermal growth factor (EGF)-like domain, a transmembrane domain, and a short cytoplasmic domain. Within the ECD, human L-selectin shares 76% and 78% amino acid sequence identity with mouse and rat L-selectin, respectively. Several isoforms arising from alternative splicing have been reported, some with potential therapeutic implications (2,3). L-selectin is constitutively expressed on a wide variety of leukocytes and plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites (1-4). Acting in cooperation with P-Selectin and E-Selectin, L-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium (5). This initial interaction, also involving ICAM-1 and VCAM-1, leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue (6). L-selectin function is required for normal Treg cell migration and over expression might be result in reduced tumor growth (7). Several studies have reported that levels of L-Selectin may be elevated or lowered in subjects with a variety of conditions, such as in Alzheimer's disease or rheumatoid arthritis (3, 5).

References

  1. Ivetic, A. et al. (2019) Front. Immunol. 10:1068.
  2. Grailer, J.J. et al. (2009) J. Dermatol sci. 56:141.
  3. Hirata, T. et al. (2015) Biochem Biophys Res. Commun. 462:371.
  4. Wedepohl, S. et al. (2012) Euro. J. Cell Biol. 91:257.
  5. Ivetic, A. et al. (2013) Inter. J. Biochem. Cell Biol. 45:550.
  6. Granger, D.N. and Senchenkova, E. (2010) Inflammation and the Microcirculation. San Rafael (CA): Morgan & Claypool Life Sciences Chapter 7.
  7. Watson, H.A. et al. (2019) Frontiers in immunology 10:1321.

Alternate Names

CD62L, hLHRc, LAM1, LECAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR, SELL, TQ1

Entrez Gene IDs

6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)

Gene Symbol

SELL

UniProt

AAH20758.1

Additional L-Selectin/CD62L Products

Product Documents for Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

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