Recombinant Human L-Selectin/CD62L His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11374-LS

R&D Systems, part of Bio-Techne
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11374-LS-100
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Key Product Details

Source

CHO

Accession #

EAW90856.1

Conjugate

Unconjugated

Applications

Bioactivity

View all L-Selectin/CD62L Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human L-Selectin/CD62L protein
Trp52-Leu332, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp52

Predicted Molecular Mass

32 kDa

SDS-PAGE

65-75 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human L-Selectin/CD62L Antibody (Catalog # AF728) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human L-Selectin/CD62L His-tag (Catalog # 11374-LS) binds with an ED50 of 0.180-1.80 ng/mL.

Scientific Data Images for Recombinant Human L-Selectin/CD62L His-tag Protein, CF

Recombinant Human L‑Selectin/CD62L His-tag Protein Binding Activity.

When Human L-Selectin/CD62L Antibody (AF728) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human L-Selectin/CD62L His-tag (Catalog # 11374-LS) binds with an ED50 of 0.180-1.80 ng/mL.

Recombinant Human L‑Selectin/CD62L His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human L‑Selectin/CD62L His-tag Protein (Catalog # 11374-LS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-75 kDa.

Formulation, Preparation and Storage

11374-LS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: L-Selectin/CD62L

L-Selectin, also known as Leukocyte adhesion molecule 1 (LAM-1) and CD62L, is a type-1 cell surface glycoprotein and cell adhesion molecule of the Selectin family (1). In humans, there are 3 Selectins, P, E, and L, and they are Ca2+ dependent lectins that help mediate the initial adhesive step during inflammation and immune surveillance (2). Mature L-Selectin consists of an extracellular domain (ECD) with a C-type lectin domain and an epidermal growth factor (EGF)-like domain, a transmembrane domain, and a short cytoplasmic domain. Within the ECD, human L-selectin shares 76% and 78% amino acid sequence identity with mouse and rat L-selectin, respectively. Several isoforms arising from alternative splicing have been reported, some with potential therapeutic implications (2,3). L-selectin is constitutively expressed on a wide variety of leukocytes and plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites (1-4). Acting in cooperation with P-Selectin and E-Selectin, L-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium (5). This initial interaction, also involving ICAM-1 and VCAM-1, leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue (6). L-selectin function is required for normal Treg cell migration and over expression might be result in reduced tumor growth (7). Several studies have reported that levels of L-Selectin may be elevated or lowered in subjects with a variety of conditions, such as in Alzheimer's disease or rheumatoid arthritis (3, 5).

References

  1. Ivetic, A. et al. (2019) Front. Immunol. 10:1068.
  2. Grailer, J.J. et al. (2009) J. Dermatol sci. 56:141.
  3. Hirata, T. et al. (2015) Biochem Biophys Res. Commun. 462:371.
  4. Wedepohl, S. et al. (2012) Euro. J. Cell Biol. 91:257.
  5. Ivetic, A. et al. (2013) Inter. J. Biochem. Cell Biol. 45:550.
  6. Granger, D.N. and Senchenkova, E. (2010) Inflammation and the Microcirculation. San Rafael (CA): Morgan & Claypool Life Sciences Chapter 7.
  7. Watson, H.A. et al. (2019) Frontiers in immunology 10:1321.

Alternate Names

CD62L, hLHRc, LAM1, LECAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR, SELL, TQ1

Entrez Gene IDs

6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)

Gene Symbol

SELL

UniProt

EAW90856.1

Additional L-Selectin/CD62L Products

Product Documents for Recombinant Human L-Selectin/CD62L His-tag Protein, CF

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Product Specific Notices for Recombinant Human L-Selectin/CD62L His-tag Protein, CF

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