Recombinant Human Laminin alpha 4 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 7340-A4
Key Product Details
Source
CHO
Accession #
Structure / Form
Monomer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Laminin alpha 4 protein
Gln826-Ala1816, with a C-terminal 6-His tag
Gln826-Ala1816, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln826 predicted, sequencing might be blocked, no result obtained
Predicted Molecular Mass
111 kDa
SDS-PAGE
110-120 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of HT1080 human fibrosarcoma cells.
The ED50 for this effect is 0.7-2.8 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
The ED50 for this effect is 0.7-2.8 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
7340-A4
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 200 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Laminin alpha 4
The alpha4 chain of laminin (LAMA4) is a secreted ~210 kDa glycoprotein that contributes to the formation of LN411 (laminin-8, subunits alpha4 beta1 gamma1), LN421 (laminin-9, subunits alpha4 beta2 gamma1), and LN423 (laminin-14, subunits alpha4 beta2 gamma3) (1-4). Laminin alpha, beta and gamma subunits interact via alpha-helical domains to create cruciform, disulfide-linked, coiled-coil laminin heterotrimers (1, 2). LAMA4 is mainly expressed in mesodermal tissues, and is produced by endothelium, cardiac and skeletal muscle fibers, lung mesenchymal cells, pre-adipocytes, kidney mesangial cells, peripheral nerves and neutrophils (3-8). It is found in the subendothelium in muscle, brain, and bone marrow, and in heart, skin, and the perineurium of peripheral nerves (3-7, 9, 10). The human LAMA4 cDNA encodes 1823 amino acids (aa), including a 24 aa signal sequence and a 1799 aa mature protein that contains three EGF-like repeats (aa 82-240) followed by five coiled-coil regions (aa 320-806) and five globular, laminin G-like (LG) domains (aa 833-1820). Potential isoforms of 1816 aa and 1791 aa are lacking sequences following the EGF-like repeats. A protease-sensitive region between LG3 and LG4 produces a processed ~180 kDa form that is detected in vivo (9, 10). Within the LG region, human and mouse LAMA4 share 91% aa sequence identity. LAMA4 LG domains serve as Ca++-mediated receptors for integrins such as alphav beta3 and alpha3 beta1 (mainly at LG2), fibulin-1 and -2 (multiple LG), and heparan sulfate proteoglycans such as syndecans 2 and 4 (mainly at LG4) (8-12). Deletion of LAMA4 in mice results in misalignment of pre- and post-synaptic membranes at neuromuscular junctions, faulty nerve myelination, chronic kidney disease, deficient neutrophil extravasation, and microvessel instability, which causes cardiomyopathy and circulatory dysfunctions (5-8, 12-14). These results show the importance of LAMA4-containing laminins in normal synapse maturation and blood vessel maturation.
References
- Tzu, J. and M.P. Marinkovich (2008) Int. J. Biochem. Cell Biol. 40:199.
- Durbeej, M. (2010) Cell Tiss. Res. 339:259.
- Liu, J. et al. (1996) Matrix Biol. 15:433.
- Iivanainen, A. et al. (1997) J. Biol. Chem. 272:27862.
- Patton, B.L. et al. (2001) Nat. Neurosci. 4:597.
- Wallquist, W. et al. (2005) J. Neurosci. 25:3692.
- Abrass, C.K. et al. (2010) Am. J. Pathol. 176:839.
- Wondimu, Z. et al. (2004) Blood 104:1859.
- Matsuura, H. et al. (2004) J. Invest. Dermatol. 122:614.
- Talts, J.F. et al. (2000) J. Biol. Chem. 275:35192.
- Gonzalez, A.M. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16075.
- DeHahn, K.C. et al. (2004) Exp. Cell Res. 294:281.
- Thyboll, J. et al. (2002) Mol. Cell Biol. 22:1194.
- Wang, J. et al. (2006) J. Biol. Chem. 281:213.
Alternate Names
LAMA4
Gene Symbol
LAMA4
UniProt
Additional Laminin alpha 4 Products
Product Documents for Recombinant Human Laminin alpha 4 Protein, CF
Product Specific Notices for Recombinant Human Laminin alpha 4 Protein, CF
For research use only
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