Recombinant Human LAMP-1/CD107a Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4800-LM

R&D Systems, part of Bio-Techne
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4800-LM-050
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Key Product Details

Source

NS0

Accession #

P11279

Conjugate

Unconjugated

Applications

Bioactivity

View all LAMP-1/CD107a Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LAMP-1/CD107a protein
Ala29-Met382 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala29 & Met30

Predicted Molecular Mass

39.2 kDa

SDS-PAGE

100-110 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LAMP1/CD107a is coated at 1 μg/mL, Recombinant Human Galectin‑3 (Catalog # 1154-GA) binds with an apparent KD <25 nM.

Formulation, Preparation and Storage

4800-LM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAMP-1/CD107a

Lysosomal associated membrane protein 1 (LAMP1), also known as CD107a and LGP120, is an approximately 120 kDa transmembrane glycoprotein that is a major protein component of lysosomal membranes (1). Mature human LAMP1 consists of a 354 amino acid (aa) intralumenal domain (ECD), a 23 aa transmembrane segment, and a 12 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O-linked glycosylation (2, 3). Within the lumenal domain, human LAMP1 shares approximately 64% aa sequence identity with mouse and rat LAMP1. The sorting of LAMP1 to lysosomes relies on a tyrosine motif in the cytoplasmic tail (3, 4). In cytotoxic T cells and mast cells, LAMP1 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (5-7). LAMP1 is presented on the plasma membrane during the activation of NK cells, CD8+ T cells, mast cells, basophils, monocytes, and platelets (6-12). A glycoform of LAMP1 known as M150 is expressed on the surface of activated macrophages where it promotes T cell costimulation and a Th1 biased immune response (13). Exposure of epithelial cells to pathogenic Neisseria bacteria induces the redistribution of LAMP1 to the cell surface where it can be cleaved by the Neisseria IgA1 protease (14). LAMP1 is a native ligand for lectins Galectin-1 and Galectin-3 (15‑17).

References

  1. Eskelinen, E.L. et al. (2003) Trends Cell Biol. 13:137.
  2. Viitala, J. et al. (1988) Proc. Natl. Acad. Sci. 85:3743.
  3. Carlsson, S.R. et al. (1988) J. Biol. Chem. 263:18911.
  4. Rohrer, J. et al. (1996) J. Cell Biol. 132:565.
  5. Peters, P.J. et al. (1991) J. Exp. Med. 173:1099.
  6. Betts, M.R. et al. (2003) J. Immunol. Meth. 281:65.
  7. Grutzkau, A. et al. (2004) Cytometry 61:62.
  8. Alter, G. et al. (2004) J. Immunol. Meth. 294:15.
  9. Tomescu, C. et al. (2009) J. Leukoc. Biol. 85:871.
  10. Hennersdorf, F. et al. (2005) Cell Res. 15:325.
  11. Kannan, K. et al. (1996) Cell. Immunol. 171:10.
  12. Fabbraio, M. and R.L. Silverstein (1990) J. Biol. Chem. 265:18531.
  13. Prasad, D.V.R. et al. (2002) J. Immunol. 169:1801.
  14. Lin, L. et al. (1997) Mol. Microbiol. 24:1083.
  15. Skrincosky, D.M. et al. (1993) Cancer Res. 53:2667.
  16. Inohara, H. and Raz, A. (1994) Biochem. Biophys. Res. Commun. 201:1366.
  17. Ohannesian D.W. et al. (1994) Cancer Res. 54:5992.

Long Name

Lysosome-associated Membrane Glycoprotein 1

Alternate Names

CD107a, LAMP1

Entrez Gene IDs

3916 (Human); 16783 (Mouse)

Gene Symbol

LAMP1

UniProt

P11279

Additional LAMP-1/CD107a Products

Product Documents for Recombinant Human LAMP-1/CD107a Protein, CF

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Product Specific Notices for Recombinant Human LAMP-1/CD107a Protein, CF

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