Recombinant Human LAR Protein

R&D Systems, part of Bio-Techne | Catalog # 9377-PF

R&D Systems, part of Bio-Techne
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9377-PF-050
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Key Product Details

Source

NS0

Accession #

P10586-2

Conjugate

Unconjugated

Applications

Bioactivity

View all LAR Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LAR protein
Ala27-Glu1251 with a C-terminal 6-His tag, and its proteolyzed forms Ala27-Arg1169 and Gln1170-Glu1251 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27 and Gln1170

Predicted Molecular Mass

136 kDa

SDS-PAGE

114-146 kDa and 14 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LRFN5 Fc Chimera (Catalog # 9385-SA) is used at 1 μg/mL, the concentration of Recombinant Human LAR that produces 50% optimal binding response is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Human LAR Protein

Recombinant Human LAR Protein Bioactivity

Recombinant Human LAR Protein Bioactivity

When Recombinant Human LRFN5 Fc Chimera (Catalog # 9385-SA) is used at 1 µg/mL, 100 µL/well, Recombinant Human LAR (Catalog # 9377-PF) binds with an ED50 of 1.5-9 µg/mL.

Formulation, Preparation and Storage

9377-PF
Formulation Supplied as a 0.2  μm filtered solution in PBS.
Reconstitution

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: LAR

PTPRF (Receptor-type tyrosine-protein phosphatase F), also called LAR (Leukocyte common antigen related), is a 212 kDa single-pass type I membrane protein that is a member of the protein tyrosine phosphatase (PTP) family and the receptor class 2A subfamily (1). Human LAR cDNA encodes 1,907 amino acids (aa) including a 29 aa signal sequence, a 1234 aa extracellular region with six potential N-glycosylation sites, a 21 aa transmembrane sequence, and a 623 aa cytoplasmic domain (1). An 1,898 aa isoform appears from alternative splicing; it is missing aa 772 to 780 (1). Human LAR shares a 95% aa sequence identity with mouse and rat LAR. LARs have been shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling (2). An increased expression level of LARs have been found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance (3). LARs have been identified as novel ligands of SALM5/LRFN-5 that mediates SALM5/LRFN-5 dependent presynaptic differentiation in a splicing- dependent manner. SALM5/LRFN-5 interacts directly with the Ig domain of LAR family receptor protein tyrosine phosphatases. The postsynaptic SALM5/LRFN-5 promotes synapse development by trans-synaptically interacting with presynaptic LARs which is important for the regulation of excitatory synaptic strength (4). Clinically, LAR has been shown down-regulated in cancers and its up-regulation is associated with better clinical outcomes (5).

References

  1. SwissProt Accession # P10586.
  2. Um J.W. et al. (2013) Trends Cell Biol. 23:465.
  3. Mander A. et al. (2005) FEBS Lett. 579:3024.
  4. Choi, Y. et al. (2016) Sci Rep. 6:26676.
  5. Bera, R. et al. (2014) Hepatology. 59:2238.

Long Name

Leukocyte Antigen-Related Tyrosine Phosphatase

Alternate Names

PTPRF

Entrez Gene IDs

5792 (Human); 104121 (Mouse)

Gene Symbol

PTPRF

UniProt

P10586-2

Additional LAR Products

Product Documents for Recombinant Human LAR Protein

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Product Specific Notices for Recombinant Human LAR Protein

For research use only

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