Recombinant Human LH alpha/beta Heterodimer Protein

R&D Systems, part of Bio-Techne | Catalog # 8899-LH

R&D Systems, part of Bio-Techne
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Carrier Free
8899-LH-010/CF
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With Carrier
8899-LH-010
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Key Product Details

Source

CHO

Accession #

P01215 ( alpha chain) & P01229 ( beta chain)

Structure / Form

Non-covalent heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all LH alpha/beta Heterodimer Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LH alpha/beta Heterodimer protein
Ala25-Ser116 ( alpha chain) & Ser21-Leu141 ( beta chain)

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25 ( alpha chain), Ser21 ( beta chain)

Predicted Molecular Mass

10 kDa ( alpha chain), 13 kDa ( beta chain)

SDS-PAGE

15-26 kDa, reducing conditions

Activity

Measured by its ability to induce cAMP accumulation in MLTC-1 mouse Leydig tumor cells. Rebois, R.V. et al. (1982) J. Cell Biol. 94:70.
The ED50 for this effect is 0.075-0.75 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8899-LH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8899-LH/CF
Formulation Lyophilized from a 0.2 μm filtered solution in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LH alpha/beta Heterodimer

Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha) is also a component of Follicle-Stimulating Hormone (FSH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein’s specific biological action and is responsible for the interaction with its receptor (1, 2). The approximately 20 kDa human CG alpha subunit shares 73% and 72% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. The approximately 18 kDa human LH beta subunit shares 71% and 72% aa sequence identity with the mouse and rat orthologs, respectively. Multiple isoforms of LH exist due to differences in the post-translational glycosylation, sialylation, and sulphation modifications of its subunits (3-6). The composition, longevity, and activity of the different LH isoforms vary throughout a woman’s menstrual cycle and reproductive life cycle (7). LH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, LH secretion can also be stimulated by estradiol (7, 8). LH works in concert with FSH to regulate female reproduction; FSH stimulates follicular growth and LH induces ovulation (9). LH also drives formation of the corpus luteum by promoting progesterone production (7). Additionally, LH has been suggested to stimulate the adrenal gland in postmenopausal women to induce secretion of sulfated DHEA, a precursor to androgens (10, 11). In the testis, LH induces Leydig cell production of testosterone (7). Hypersecretion of LH has been shown to occur in women with polycystic ovary syndrome and is associated with an increased risk of infertility and miscarriage (12, 13). Additionally, increased serum LH levels are associated with decreased cognition and have been implicated in the development and progression of Alzheimer’s disease (14, 15).

References

  1. Keutmann, H.T. et al. (1987) Proc. Natl. Acad. Sci. USA 84:2038.
  2. Keutmann, H.T. et al. (1992) Mol. Endocrinol. 6:904.
  3. Wilson, C.A. et al. (1990) J. Endocrinol. 125:3.
  4. Stanton, P.G. et al. (1993) J. Endocrinol. 138:529.
  5. Stanton, P.G. et al. (1996) Mol. Cell. Endocrinol. 125:133.
  6. Bergendah, M. and J.D. Veldhuis (2001) Hum. Reprod. 16:1058.
  7. Choi, J. and J. Smitz (2014) Mol. Cell. Endocrinol. 383:203.
  8. Marshall, J.C. et al. (1993) Trans. Am. Clin. Climatol. Assoc. 104:31.
  9. Raju, G.A. et al. (2013) J. Hum. Reprod. Sci. 6:227.
  10. Alevizaki, M. et al. (2006) Eur. J. Endocrinol. 154:875.
  11. Moran, F.M. et al. (2013) Menopause 20:329.
  12. Balen, A.H. (1995) J. R. Soc. Med. 88:339P.
  13. Blank, S.K. et al. (2006) Hum. Reprod. Update 12:351.
  14. Webber, K.M. et al. (2007) Clin. Med. Res. 5:177.
  15. Blair, J.A. et al. (2015) Front. Endocrinol. (Lausanne) 6:45.

Long Name

Luteinizing Hormone alpha/beta Heterodimer

UniProt

P01215 ( alpha chain) & P01229 ( beta chain)

Additional LH alpha/beta Heterodimer Products

Product Documents for Recombinant Human LH alpha/beta Heterodimer Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8899-LH (with carrier)
Download SDS 8899-LH/CF (carrier free)

Product Specific Notices for Recombinant Human LH alpha/beta Heterodimer Protein

For research use only

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