Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9226-T4

R&D Systems, part of Bio-Techne
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9226-T4-050
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Key Product Details

Source

NS0

Accession #

O75019

Conjugate

Unconjugated

Applications

Bioactivity

View all LILRA1/CD85i/LIR-6 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LILRA1/CD85i/LIR-6 protein
Pro17-Asn461, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro17 & Gly24

Predicted Molecular Mass

49 kDa

SDS-PAGE

62-76 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 = 0.3-1.8 µg/mL.

Reviewed Applications

Read 1 review rated 5 using 9226-T4 in the following applications:

Scientific Data Images for Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

Recombinant Human LILRA1/CD85i/LIR-6 Protein Bioactivity

Recombinant Human LILRA1/CD85i/LIR-6 Protein Bioactivity

When Recombinant Human LILRA1/CD85i/LIR-6 (Catalog # 9226-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 of 0.3-1.8 µg/mL.

Formulation, Preparation and Storage

9226-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA1/CD85i/LIR-6

LILRA1, also known as CD85i and LIR-6, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA1 consists of a 445 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail (2). The transmembrane segment contains a positively charged arginine which may mediate association with signaling molecules. Alternative splicing generates an isoform that lacks the third and fourth Ig-like domains (2). LILRA1 is expressed on monocytes (3, 4) and binds to the free heavy chain of the MHC class I molecule HLA-B27 (4, 5). It is activated in response to the BCG mycobacterial strain (6). R&D Systems in-house testing indicates that LILRA1 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (7).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Borges, L. et al. (1997) J. Immunol. 159:5192.
  3. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  4. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  5. Jones, D.C. et al. (2011) J. Immunol. 186:2990.
  6. Hogan, L.E. et al. (2016) Sci Rep. 6:21780.
  7. Zheng, J. et al. (2012) Nature 485:656.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1

Alternate Names

CD85i, LIR-6, LIR6

Entrez Gene IDs

11024 (Human)

Gene Symbol

LILRA1

UniProt

O75019

Additional LILRA1/CD85i/LIR-6 Products

Product Documents for Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human LILRA1/CD85i/LIR-6 Protein, CF

For research use only

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