Recombinant Human LILRA3/CD85e Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9517-T4

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

Q8N6C8-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all LILRA3/CD85e Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LILRA3/CD85e protein
Human LILRA3/CD85e
(Gly24-Glu439)
Accession # Q8N6C8-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

72 kDa

SDS-PAGE

84-98 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Angiopoietin‑like Protein 7/ANGPTL7 (Catalog # 914-AN) is coated onto a microplate at 2 µg/mL, Recombinant Human LILRA3/CD85e Fc Chimera (Catalog # 9517-T4) binds with an ED50 of 7-42 ng/mL.

Scientific Data Images for Recombinant Human LILRA3/CD85e Fc Chimera Protein, CF

Recombinant Human LILRA3/CD85e Fc Chimera Protein Bioactivity

Recombinant Human LILRA3/CD85e Fc Chimera Protein Bioactivity

When Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) is coated onto a microplate at 2 µg/mL, Recombinant Human LILRA3/CD85e Fc Chimera (Catalog # 9517-T4) binds with an ED50 = 7-42 ng/mL.

Formulation, Preparation and Storage

9517-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA3/CD85e

Human LILRA3, also known as Leukocyte Ig-like receptor 4 (LIR-4), CD85 antigen-like family member E, Immunogobulin-like transcript 6 (ILT-6) and monocyte inhibitory receptor HM43/HM31,  is a 70 kD Ig superfamily member that belongs to the leukocyte receptor complex/cluster. Mature LILRA3 is 416 amino acids (aa) in length and contains four C2-type Ig-like domains (1, 2). Unlike other LILR family members, LILRA3 is actively secreted (3). Alternative splicing generates additional isoforms with  one containing an extra 18 aa in the signal peptide and/or a 64 amino acid deletion in the C-terminal half of the second Ig-like domain (4). Human LILRA3 shares ~86% aa identity with Chimpanzee LILRA3. Human LILRA3 acts as a soluble receptor for class I MHC antigens. It binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. It also binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes (5). R&D Systems in-house testing indicates that LILRA3 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (6).

References

  1. Arm, J.P. et.al. (1997). J. Immunol. 159:2342.
  2. Ryu, M. et. al. (2011) PLoS One. 6:e19245.
  3. Martin, A.M. et al. (2002) Trends Immunol. 23:81
  4. Samaridis, J. & M. Colonna (1997) Eur. J. Immunol. 27:660.
  5. Lee T.H. et. al. (2013). J. Biol. Chem. 288:32873.
  6. Zheng, J. et al. (2012) Nature 485:656.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM and ITIM Domains), Member 3

Alternate Names

CD85e, HM31, HM43, ILT6, LIR4

Entrez Gene IDs

11026 (Human)

Gene Symbol

LILRA3

UniProt

Q8N6C8-1

Additional LILRA3/CD85e Products

Product Documents for Recombinant Human LILRA3/CD85e Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human LILRA3/CD85e Fc Chimera Protein, CF

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