Recombinant Human LILRA6/CD85b/ILT8 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9088-T4

R&D Systems, part of Bio-Techne
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9088-T4-050
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Key Product Details

Source

HEK293

Accession #

Q6PI73

Conjugate

Unconjugated

Applications

Bioactivity

View all LILRA6/CD85b Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LILRA6/CD85b protein
Gly24-Asn447, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

47 kDa

SDS-PAGE

62-69 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LILRA6/CD85b/ILT8 is coated onto a microplate at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 = 0.1-0.6 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 9088-T4 in the following applications:

Scientific Data Images for Recombinant Human LILRA6/CD85b/ILT8 Protein, CF

Recombinant Human LILRA6/CD85b/ILT8 Protein Bioactivity

Recombinant Human LILRA6/CD85b/ILT8 Protein Bioactivity

When Recombinant Human LILRA6/CD85b/ILT8 is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7 (Catalog # 914-AN) binds with a typical ED50 of 0.1-0.6 µg/mL.

Formulation, Preparation and Storage

9088-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA6/CD85b

LILRA6, also known as ILT8 and CD85b, is a transmembrane molecule that belongs to the LILR family of immune regulatory proteins (1, 2). Among family members, LILRA6 is most closely related to LILRB3/CD85a/ILT5; these proteins share 94% amino acid (aa) sequence identity in their extracellular domains (ECD). Mature human LILRA6 consists of a 424 aa ECD with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 13 aa cytoplasmic domain (3). It contains a positively charged arginine residue in the transmembrane segment which may mediate association with the signaling protein Fc epsilon RI gamma. Alternative splicing generates a short isoform that is truncated near the middle of the ECD. LILRA6 is highly polymorphic, and its gene is found in variably copy numbers (4, 5). LILRA6 is primarily expressed on monocytes (4). R&D Systems in-house testing indicates that LILRA6 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (6).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Hirayasu, K. and H. Arase (2015) J. Hum. Genet. 60:703.
  3. Wende, H. et al. (2000) Immunogenetics 51:703.
  4. Bashirova, A.A. et al. (2014) Immunogenetics 66:1.
  5. Lopez-Alvarez, M.R. et al. (2014) Immunogenetics 66:73.
  6. Zheng, J. et al. (2012) Nature 485:656.

Long Name

Leukocyte Immunoglobulin-like Receptor A6

Alternate Names

7M1, CD85b, ILT8, Pira3

Entrez Gene IDs

79168 (Human); 18726 (Mouse)

Gene Symbol

LILRA6

UniProt

Q6PI73

Additional LILRA6/CD85b Products

Product Documents for Recombinant Human LILRA6/CD85b/ILT8 Protein, CF

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Product Specific Notices for Recombinant Human LILRA6/CD85b/ILT8 Protein, CF

For research use only

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