Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

Name: Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF
Brand: R&D Systems
SKU: BT10429

R&D Systems, part of Bio-Techne | Catalog # BT10429

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Product Datasheet / COA / SDS

Fc Chimera Biotinylated

Key Product Details

Source:	HEK293
Structure / Form:	Biotinylation via amines
Applications:	Bioactivity

View Full Technical Details for Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human LILRB4/CD85k/ILT3 protein

Human LILRB4 (Gly24-Glu259) Accession # NP_001265355.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly24

Predicted Molecular Mass

53 kDa

SDS-PAGE

53 kDa

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera binds to Human Fibronectin Protein (Catalog # 1918-FN) with an ED₅₀ of 0.400-4.00 μg/mL.

Scientific Data Examples for Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

	Biotinylated Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein Binding Activity. Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein (Catalog # BT10429) binds to Human Fibronectin Protein (1918-FN) with an ED₅₀ of 0.400-4.00 μg/mL.
	Biotinylated Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein (Catalog # BT10429) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa and 100-140 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT10429

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: LILRB4/CD85k/ILT3

ILT3, also known as LILRB4, CD85k, and LIR5, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation (1). Mature human ILT3 consists of an extracellular domain (ECD) with two Ig-like domains, a transmembrane segment, and a cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). The mature ECD of human ILT3 shares 44% amino acid identity with mouse ILT3. Alternative splicing of human ILT3 generates an isoform that lacks the first ITIM and a secreted isoform that circulates in the serum of cancer patients (3, 4). ILT3 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC) (2, 5, 6). Ligation of ILT3 triggers ITIM-mediated inhibition of cell-activating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection (2, 4, 7, 8). Soluble ILT3 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL (4, 7, 9). In turn, CD8+ Ts cells induce ILT3 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC (5, 6, 8, 10, 11). Recently, a novel anti-LILRB4 CAR-T Cell was been used to treat monocytic acute myeloid leukemia in humanized hematopoietic-reconstituted mice models (12).

Item	Value
References	Vlad, G. et al. (2010) Int. Rev. Immunol. 29:119. Cella, M. et al. (1997) J. Exp. Med. 185:1743. Heinzmann, A. et al. (2000) Eur. J. Immunogenet. 27:121. Suciu-Foca, N. et al. (2007) J. Immunol. 178:7432. Gleissner, C.A. et al. (2007) Eur. J. Immunol. 37:177. Manavalan, J.S. et al. (2004) Int. Immunol. 16:1055. Vlad, G. and N. Suciu-Foca (2012) Exp. Mol. Pathol. 93:294. Chang, C.C. et al. (2002) Nat. Immunol. 3:237. Vlad, G. et al. (2006) Int. Immunopharmacol. 6:1889. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245. Brenk, M. et al. (2009) J. Immunol. 183:145. John, S. et al. (2018) Mol. Ther. 26:2487.
Long Name	Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
Entrez Gene IDs	11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)
Alternate Names	CD85 antigen-like family member K, CD85k, CD85k antigen, HM18, ILT-3, ILT3, ILT3CD85K, LILRB4, LIR-5subfamily B, member 4, LIR5, LIR5LILRB5, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4

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Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT10429

Key Product Details

Product Specifications for Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

Scientific Data Examples for Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: LILRB4/CD85k/ILT3

Customer Reviews for Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

Product Documents for Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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