Recombinant Human LINGO-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9737-LG

R&D Systems, part of Bio-Techne
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9737-LG-050
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Key Product Details

Source

HEK293

Accession #

Q96FE5-1

Conjugate

Unconjugated

Applications

Bioactivity

View all LINGO-1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LINGO-1 protein
Thr40-Thr556, with a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr40

Predicted Molecular Mass

60 kDa

SDS-PAGE

82-112 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human Nogo Receptor Fc Chimera is present at 2.5 μg/mL, the concentration of Recombinant Human LINGO-1 that produces 50% of the optimal binding response is 0.4-2.4 μg/mL.  

Scientific Data Images for Recombinant Human LINGO-1 Protein, CF

Recombinant Human LINGO-1 Protein Binding Activity

Recombinant Human LINGO-1 Protein Binding Activity

When Biotinylated Recombinant Human Nogo Receptor Fc Chimera is present at 2.5 μg/mL, Recombinant Human LINGO-1 (Catalog # 9737-LG) binds with an ED50 of 0.4-2.4 μg/mL.

Formulation, Preparation and Storage

9737-LG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 1 mg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: LINGO-1

LINGO-1 (Leucine-rich repeat and immunoglobulin-like domain-containing Nogo receptor-interacting protein 1), also known as LERN1 (Leucin-rich repeat neuronal protein 1) or LRRN6A (Leucine-rich repeat neuronal protein 6A), is a 614 amino acid (aa) transmembrane protein of the leucine-rich repeat (LRR) family, ribonuclease inhibitor subfamily (1). The four known LINGO proteins (LINGO-1, -2, -3 and -4) contain LRR and IgCAM domains in the extracellular portion and share 44‑61% aa identity (2). LINGO-1 is restricted to the nervous system and is concentrated in the brain as a component of the NgR1/p75 and NgR1/Taj (TROY) signaling complexes (3). LINGO-1 negatively regulates neurite outgrowth and myelination. LINGO-1 is highly conserved, showing 99% aa identity between human, mouse and rat (2, 4). LINGO-1 promotes lysosomal degradation of amyloid-beta protein precursor and acts as a key player in Alzheimer's Disease progression (5). It suppresses oligodendrocyte precursor cell and myelin maturation, which leads to neurodegeneration (6). The intracellular LINGO-1 negatively regulates Trk receptors' signaling by modulating their lysosomal degradation (7). Inhibition of LINGO-1 may protect against degeneration of dopamine neurons in Parkinson's disease (8).

References

  1. Mosyak, L. et al. (2006) J. Biol. Chem. 281:36378.
  2. Mi, S. et al. (2004) Nat. Neurosci. 7:221.
  3. Fernandez-Enright, F. et al. (2014) Transl. Psychiatry. 4:e348.
  4. Mi, S. (2008) Cytokine Growth Factor Rev. 19:245.
  5. de Laat, R. et al. (2015) Pathobiol. Aging Age Relat. Dis. 5:25796.
  6. Foale, S. et al. (2017) Neural. Regen. Res. 12:1247.
  7. Meabon, J.S. et al. (2016) Mol. Cell Neurosci. 70:1.
  8. Inoue, H. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104:14430.

Long Name

Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 1

Alternate Names

LERN1, LINGO1, LRRN6A

Entrez Gene IDs

84894 (Human)

Gene Symbol

LINGO1

UniProt

Q96FE5-1

Additional LINGO-1 Products

Product Documents for Recombinant Human LINGO-1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human LINGO-1 Protein, CF

For research use only

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