Recombinant Human Lipocalin-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1708-PI

R&D Systems, part of Bio-Techne
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1708-PI-050
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Key Product Details

Source

NS0

Accession #

P31025

Conjugate

Unconjugated

Applications

Inhibition Activity

View all Lipocalin-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Lipocalin-1 protein
His19-Asp176, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His19

Predicted Molecular Mass

19 kDa

SDS-PAGE

20-22 kDa, reducing conditions

Activity

Measured by its ability to inhibit active Cathepsin V cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The IC50 value is <400 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1708-PI
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Lipocalin-1

Lipocalin-1, also known as tear prealbumin or von Ebner’s gland protein (VEGP), is encoded by the LCN1 gene (1-3). It is a member of the Lipocalin superfamily that binds many different classes of lipophylic chemicals (4). Lipocalin-1 contains three sequence motifs similar to the cystatins, a superfamily of cysteine protease inhibitors (5). In fact, it has been suggested that Lipocalin-1 is a physiological inhibitor of cysteine proteases and plays a role in the control of inflammatory processes in oral and ocular tissues (5). Recombinant Human Lipocalin-1 corresponds to the mature and secreted protein. It is a weak inhibitor of cysteine proteases such as cathepsin V, which is similar to recombinant human Cystatin S (Catalog # http://www.rndsystems.com/product_results.aspx?k=1296-PI">1296-PI).

References

  1. Redl, B. et al. (1992) J. Biol. Chem. 267:20282.
  2. Blaker, M. et al. (1993) Biochim. Biophys. Acta 1172:131.
  3. Lassagne, H. and A.M. Gachon (1993) Exp. Eye Res. 56:605.
  4. Redl, B. et al. (2000) Biochim. Biophys. Acta 1482:241.
  5. van’t Hof, W. et al. (1997) J. Biol. Chem. 272:1837.

Alternate Names

LCN1, Lipocalin1, Tear Prealbumin, VEGP

Entrez Gene IDs

3933 (Human)

Gene Symbol

LCN1

UniProt

P31025

Additional Lipocalin-1 Products

Product Documents for Recombinant Human Lipocalin-1 Protein, CF

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Product Specific Notices for Recombinant Human Lipocalin-1 Protein, CF

For research use only

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