Recombinant Human Livin alpha Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1161-LV
Key Product Details
Source
E. coli
Accession #
Structure / Form
Monomer
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
E. coli-derived human Livin protein
Met1-Ser298, with a C-terminal 6-His tag
Met1-Ser298, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.5 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met1
Predicted Molecular Mass
34 kDa
SDS-PAGE
38 kDa, reducing conditions
Activity
Measured by its ability to reverse the inhibition of DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP.
The ED50 for this effect is 0.5-2.0 µM.
Optimal dilutions should be determined by each laboratory for each application.
The ED50 for this effect is 0.5-2.0 µM.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
1161-LV
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Livin
Livin alpha is a member of the inhibitor of apoptosis (IAP) protein family and contains a single BIR and RING finger motif. The anti-apoptotic protein appears to inhibit the activation of Caspase-9 in cell extracts activated by cytochrome c and dATP. Livin alpha is the longer of two splice variants, encoding an additional 18 amino acid residues in the BIR-RING linking region.
References
- Kasof, G.M. and B.C. Gomes (2001) J. Biol. Chem. 276(5):3238.
- Vucic, D. et al. (2000) Curr. Biol. 10:1359.
- Ashhab, Y. et al. (2001) FEBS 495:56.
- Lin, J-H. et al. (2000) Biochem. Biophys. Res. Comm. 279:820.
Alternate Names
BIRC7, KIAP, ML-IAP
Entrez Gene IDs
79444 (Human)
Gene Symbol
BIRC7
UniProt
Additional Livin Products
Product Documents for Recombinant Human Livin alpha Protein, CF
Product Specific Notices for Recombinant Human Livin alpha Protein, CF
For research use only
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