Recombinant Human LRRC52 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10260-LR

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
10260-LR-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

HEK293

Accession #

Q8N7C0

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all LRRC52 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LRRC52 protein
Human LRRC-52
(Ser24-Asp244)
Accession # Q8N7C0		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser24

Predicted Molecular Mass

52 kDa

SDS-PAGE

60-80 kDa, under reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human LRRC52 Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Scientific Data Images for Recombinant Human LRRC52 Fc Chimera Protein, CF

Recombinant Human LRRC52 Fc Chimera Protein SDS-PAGE

Recombinant Human LRRC52 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human LRRC52 Fc Chimera (Catalog # 10260-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa and 120 -140 kDa, respectively.

Formulation, Preparation and Storage

10260-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC52

LRRC52 (Leucine-rich repeat-containing protein 52) belongs to an extracellular leucine-rich-repeat–only (Elron) cluster, which includes five other LRRC proteins: LRRC26, LRRC38, LRRC55, LRTM1 and LRTM2 (1). Elron cluster members are type I transmembrane proteins with an extracellular domain (ECD) containing several LRR motifs and a cytoplasmic C-terminal tail with a short stretch of acidic residues (1). The mature ECD of LRRC52 is 221 amino acids (aa), containing 5 LRR motifs, and shares 76% and 78% identity with mouse and rat LRRC52, respectively. LRRC-52 is dominantly expressed in testis and skeletal muscle and is also detected in several other tissues including placenta, kidney, lung, and some glands (2, 3). LRRC52 has been identified as a testes specific SLO3 K+ channel accessory protein in mouse (2). LRRC52 is proposed to be essential for alkalization dependent activation of the KSper current at physiological membrane potentials and pH (2). Activation of the SLO3 channel is critical in the fertilization process and loss of function results in infertility (2, 4, 5). A second Elron cluster protein, LRRC26, was recently identified as a regulatory subunit of SLO1 BK-type K+ channels, suggesting a general function for the entire family (6). Based on function, LRRC52 might be a therapeutic target for male contraception (7).

References

  1. Dolan, J. et al. (2007) BMC Genomics 8:320.
  2. Yang, C. et al. (2011) PNAS 108:19419.
  3. Leonetti, M. et al. (2012) PNAS. 109:19274.
  4. Navarro, B. et al. (2007) PNAS 104:7688.
  5. Sanit, C. et al. (2010) FEBS Lett. 584:1041.
  6. Yan, J. and Aldrich, RW. (2010) Nature 466:513.
  7. Zeng, XH. et al. (2015) PNAS 112:2599.

Long Name

Leucine-rich Repeat Containing 52

Alternate Names

BK Channel Auxiliary Gamma Subunit LRRC52

Entrez Gene IDs

440699 (Human); 240899 (Mouse); 289199 (Rat)

Gene Symbol

LRRC52

UniProt

Q8N7C0

Additional LRRC52 Products

Product Documents for Recombinant Human LRRC52 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human LRRC52 Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.