Recombinant Human LRRTM2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5589-LR

R&D Systems, part of Bio-Techne
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5589-LR-050
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Key Product Details

Source

HEK293

Accession #

O43300

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all LRRTM2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LRRTM2 protein
Human LRRTM2
(Cys34-Arg422)
Accession # O43300
 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys34

Predicted Molecular Mass

71 kDa

SDS-PAGE

83-100 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat Neurexin 1 alpha (-S4) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human LRRTM2 Fc Chimera that produces 50% of the optimal binding response is 0.07-0.42 μg/mL.

Scientific Data Images for Recombinant Human LRRTM2 Fc Chimera Protein, CF

Recombinant Human LRRTM2 Fc Chimera Protein Binding Activity

Recombinant Human LRRTM2 Fc Chimera Protein Binding Activity

When Recombinant Rat Neurexin 1 alpha (-S4) is coated at 1 μg/mL (100 μL/well), Recombinant Human LRRTM-2 Fc Chimera (Catalog # 5589-LR) binds with an ED50 of 0.07-0.42 μg/mL
Recombinant Human LRRTM2 Fc Chimera Protein SDS-PAGE

Recombinant Human LRRTM2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human LRRTM2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-100 kDa and 160-200 kDa, respectively.

Formulation, Preparation and Storage

5589-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: LRRTM2

LRRTM2 (Leucine-rich repeat transmembrane protein 2) is a member of the LRRTM family of molecules (1). All LRRTMs are type I transmembrane proteins that contain multiple leucine rich repeats and one PDZ consensus cytoplasmic binding domain. The LRRTM family is expressed in the central nervous system across vertebrate species, and they are not found in invertebrates (1). Human LRRTM2 is synthesized as a 516 amino acid (aa) precursor that contains a 33 aa signal sequence, a 389 aa extracellular domain, a 21 aa transmembrane segment, and a 73 aa cytoplasmic region (1). The extracellular domain is characterized by the presence of ten Leucine-rich repeats, flanked by two cysteine-rich sequences. Mature human LRRTM2 is 98% aa identical to mouse LRRTM2 (1). LRRTM2 functions as a postsynaptic organizer in excitatory synapses. LRRTM2 binds only Neurexin-alpha and Neurexin-beta which are lacking splice site 4 (S4) (2). Crystal structure shows dependence of this interaction on Calcium ion as well as overlapping binding interface with Neuroligins (3). LRRTM2 is essential for long term potentiation in hippocampal neuron by maintaining AMPA Receptors at the synapse (4).

References

  1. Lauren, J. et al. (2003) Genomics 81:411.
  2. Ko, J. et al. (2009) Neuron 64:791.
  3. Yamagata, A. et al. (2018) Nat. Commun. 9:3964.
  4. Soler-Llavina, G.J. et al. (2013) Neuron 79:439.

Long Name

Leucine Rich Repeat Transmembrane Neuronal 2

Alternate Names

LRRN2

Entrez Gene IDs

26045 (Human); 107065 (Mouse); 685472 (Rat)

Gene Symbol

LRRTM2

UniProt

O43300

Additional LRRTM2 Products

Product Documents for Recombinant Human LRRTM2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human LRRTM2 Fc Chimera Protein, CF

For research use only

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