Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI8884

Biotinylated His-tag
R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AVI8884-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P01374 (alpha) & Q06643-1 (beta)

Structure / Form

GS-linked heterotrimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Lymphotoxin Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Lymphotoxin protein
Human Lymphotoxin alpha
(Leu35-Leu205)
Accession # P01374		 GGGGS

Human Lymphotoxin beta
(Gln49-Gly244)
Accession # Q06643-1 

 GGGGS 

Human Lymphotoxin beta
(Gln49-Gly244)
Accession # Q06643-1 

 HHHHHH, Avi-tag 
N-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu35

Predicted Molecular Mass

63 kDa

SDS-PAGE

69-80 kDa

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Lymphotoxin beta R Fc Chimera (Catalog # 629-LR) is immobilized at 25 ng/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) that produces 50% of the optimal binding response is 0.75-4.5 ng/mL.

Scientific Data Images for Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein, CF

Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein Binding Activity

Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein Binding Activity

When Recombinant Human Lymphotoxin betaR/TNFRSF3 Fc Chimera (Catalog # 629-LR) is immobilized at 25 ng/mL, 100 µg/well, Biotinylated Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) binds with an ED50 of 0.75-4.5 ng/mL.
Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein SDS-PAGE

Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein SDS-PAGE

2 μg/lane of Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 69-80 kDa.

Formulation, Preparation and Storage

AVI8884
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin

Lymphotoxin alpha (LT-alpha), and Lymphotoxin beta (LT-beta) are pro-inflammatory TNF superfamily ligands that play important roles in immune system development (1, 2). The 25 kDa mature human LT-alpha is a secreted protein that shares 75% amino acid (aa) sequence identity with mouse and rat LT-alpha (3, 4). The 33 kDa mature human LT-beta is a type II transmembrane protein that shares 73% aa sequence identity with mouse and rat LT-beta within common regions of their extracellular domains (5). Relative to the human protein, mouse and rat LT-beta have a 66 aa or 65 aa insertion within the ECD, respectively. LT-alpha can be secreted as a homotrimer that binds and activates TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, HVEM/TNFRSF14, and Troy/TNFRSF19 (6-8). LT-alpha is required for development of the spleen, lymph nodes, and Peyer’s patches (9). It also regulates T cell homing to the gut and IgA induction (10). In addition, LT-alpha can form membrane-associated heterotrimers with two copies of LT-beta on the surface of B, T, LTi, and ILC3 cells (2, 5, 11). The Lymphotoxin alpha1/ beta2 heterotrimer binds and activates the Lymphotoxin beta R/TNFRSF3 (LT betaR) which is expressed on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules (HEV) (2, 12, 13). LT betaR also serves as a receptor for LIGHT/TNFSF14 (14). LT-alpha 1/ beta2 promotes the development of FDC networks and HEV in lymphoid tissue, the class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by ILCs (10, 15-17). It can be shed by ADAM17 or MMP-8 mediated cleavage, and the released heterotrimer circulates in the serum and is elevated in synovial fluid of rheumatoid arthritis patients (18).

References

  1. Lu, T.T. and J.L. Browning (2014) Front. Immunol. 5:47.
  2. Upadhyay, V. and Y.-X. Fu (2014) Cytokine Growth Factor Rev. 25:227.
  3. Gray, P.W. et al. (1984) Nature 312:721.
  4. Nedwin, G.E. et al. (1985) J. Cell Biochem. 29:171.
  5. Browning, J.L. et al. (1993) Cell 72:847.
  6. Schoenfeld, H.J. et al. (1991) J. Biol. Chem. 266:3863.
  7. Mauri, D.N. et al. (1998) Immunity 8:21.
  8. Hashimoto, T. et al. (2008) Cell Cycle 7:106.
  9. De Togni, P. et al. (1994) Science 264:703.
  10. Kruglov, A.A. et al. (2013) Science 342:1243.
  11. Androlewicz, M.J. et al. (1992) J. Biol. Chem. 267:2542.
  12. Sudhamsu, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:19896.
  13. Crowe, P.D. et al. (1994) Science 264:707.
  14. Eldredge, J. et al. (2006) Biochemistry 45:10117.
  15. Futterer, A. et al. (1998) Immunity 9:59.
  16. Koni, P.A. et al. (1997) Immunity 6:491.
  17. Ota, N. et al. (2011) Nat. Immunol. 12:941.
  18. Young, J. et al. (2010) Cytokine 51:78.

Alternate Names

LT, Lymphotoxin alpha, tnfb, TNFSF1, TNLG1E

Entrez Gene IDs

4049 (Human); 16992 (Mouse); 102138497 (Cynomolgus Monkey)

Gene Symbol

LTA

UniProt

P01374 (alpha) & Q06643-1 (beta)

Additional Lymphotoxin Products

Product Documents for Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Lymphotoxin alpha1/beta2 Avi Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.