Recombinant Human Marapsin/Pancreasin Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1988-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked heterodimer
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Marapsin/Pancreasin protein
Ala23-Arg34, and Met35-Lys290 with a C-terminal 10-His tag
Ala23-Arg34, and Met35-Lys290 with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala23 & Met35
Predicted Molecular Mass
31 kDa
SDS-PAGE
39 kDa, reducing conditions
Activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >2,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >2,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1988-SE
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM MES and 100 mM NaCl, pH 6.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Marapsin/Pancreasin
Marapsin, pancreasin, and channel-activating protease 2 (CAP-2) are different names for the same serine protease that is expressed strongly in the pancreas (1, 2). The protein is synthesized with a signal peptide (amino acid residues 1-22), a pro peptide (residues 23-34) and a mature chain (residues 35-290) corresponding to the serine protease domain. The full-length protein was expressed and the secreted protein purified. The N-terminal sequencing results indicate that the purified protein is a disulfide bond-linked dimer formed between the pro peptide and the mature chain. The active enzyme has low activity against peptide substrates tested, but high activity against thioester substrates. The peptidase activity is inhibited by 20 mM benzamidine.
References
- Bhagwandin, V.J. et al. (2003) J. Biol. Chem. 278:3363.
- Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
Alternate Names
CAP-2, MPN, Pancreasin, PRSS27
Gene Symbol
PRSS27
UniProt
Additional Marapsin/Pancreasin Products
Product Documents for Recombinant Human Marapsin/Pancreasin Protein, CF
Product Specific Notices for Recombinant Human Marapsin/Pancreasin Protein, CF
For research use only
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