Recombinant Human MDGA1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11368-MD

R&D Systems, part of Bio-Techne
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11368-MD-050
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Key Product Details

Source

HEK293

Accession #

Q8NFP4.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all MDGA1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human MDGA1 protein
Human MDGA1
(Gln19-Gly931)
Accession # Q8NFP4.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln19 predicted

Predicted Molecular Mass

128 kDa

SDS-PAGE

125-145 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human MDGA1 Fc Chimera (Catalog # 11368-MD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Neuroligin 2/NLGN2  (Catalog # 5645-NL) binds with an ED50 of 0.125-1.50 µg/mL.

Scientific Data Images for Recombinant Human MDGA1 Fc Chimera Protein, CF

Recombinant Human MDGA1 Fc Chimera Protein Binding Activity.

When Recombinant Human MDGA1 Fc Chimera Protein (Catalog # 11368-MD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Neuroligin 2/NLGN2 (5645-NL) binds with an ED50 of 0.125-1.50 µg/mL.

Recombinant Human MDGA1 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human MDGA1 Fc Chimera Protein (Catalog # 11368-MD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 125-145 kDa and 250-290 kDa, respectively.

Formulation, Preparation and Storage

11368-MD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Background: MDGA1

MDGA1 (MAM domain containing glycosylphosphatidylinositol anchor 1) is a 135‑140 kDa glycoprotein within the IgCAM superfamily of adhesion molecules (1‑3). MDGA1 is thought to mediate neuronal migration, neurite outgrowth, and cell-cell adhesion within the central and peripheral nervous system (1, 4‑8). Human MDGA1 precursor is a 955 amino acid (aa) protein that produces a 914 aa mature protein with six Ig-like domains, a fibronectin type III domain (aa 640-739), a MAM domain (aa 751-918), and a GPI anchor (aa 932) (1-3). Mature human MDGA1 shares 95%, 96%, and 98% aa sequence identity with mature mouse, rat, and canine MDGA1, respectively. Potential human variants of 973, 904 and 587 aa contain a 48 aa substitution for aa 936-955, a 58 aa substitution for aa 847-955, and a 51 aa substitution for aa 537-955, respectively. Human MDGA1 also shares 54% aa sequence identity with human MDGA2, and may also share some functional redundancy (1, 4). MDGA1 is mainly expressed on restricted populations of neurons in the central and peripheral nervous system, such as embryonic neurons destined for cortical layers 2/3, migrating basilar pontine neurons and D1 interneurons of the spinal cord (1, 5‑7). Deletion or down‑regulation of mouse MDGA1 slows radial migration of neurons, indicating a role for MDGA1 in radial migration of cortical neurons (4, 5). MAM and Ig-like domains are involved in heterophilic and homophilic adhesion (7, 8). MDGA1 expression is also reported on primary cells and cell lines from leukemias, lymphomas, and tumors of the lung, colon, uterus, stomach and breast (3).

References

  1. Litwack, E.D. et al. (2004) Mol. Cell. Neurosci. 25:263.
  2. Diaz-Lopez, A. et al. (2005) Exp. Cell Res. 307:91.
  3. De Juan, C. et al. (2002) Oncogene 21:3089.
  4. Ishikawa, T. et al. (2011) Dev. Dyn. 240:96.
  5. Takeuchi, A. and D.D.M. O'Leary (2006) J. Neurosci. 26:4460.
  6. Takeuchi, A. et al. (2007) Cereb Cortex. 17:1531.
  7. Fujimura, Y. et al. (2006) Brain Res. 1101:12.
  8. Diaz-Lopez, A. et al. (2011) Cancer Microenviron. 4:23.

Long Name

MAM Domain Containing Glycosylphosphatidylinositol Anchor 1

Alternate Names

GPIM, MAMDC3

Entrez Gene IDs

266727 (Human); 74762 (Mouse)

Gene Symbol

MDGA1

UniProt

Q8NFP4.1

Additional MDGA1 Products

Product Documents for Recombinant Human MDGA1 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human MDGA1 Fc Chimera Protein, CF

For research use only

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