Recombinant Human Megalin/LRP-2 (C3) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9578-LR

R&D Systems, part of Bio-Techne
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9578-LR-020
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Key Product Details

Source

NS0

Accession #

P98164

Conjugate

Unconjugated

View all Megalin/LRP2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Megalin/LRP2 protein
Glu2698-Gly3110, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu2698

Predicted Molecular Mass

47 kDa

SDS-PAGE

82-95 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9578-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Megalin/LRP2

Megalin, also known as the low-density lipoprotein receptor-related protein 2 (LRP2), is a large type I transmembrane cell surface protein. This glycoprotein is a multi‑ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney (1). The Megalin protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling. Mutations in this gene cause Donnai-Barrow Syndrome (DBS) and Facio-Oculoacoustico-Renal Syndrome (FOAR) (1). Megalin is consisting of a 25 amino acid (aa) probable N‑terminal signal peptide sequence, a 4400 aa extracellular region, a 22 aa single transmembrane domain, and a 213 aa C‑terminal cytoplasmic tail. The entire extracellular region is made up of 36 class A motifs of putative ligand-binding domains arranged in four distinct clusters, 16 growth factor repeats separated by 8 YWTD spacer regions, and 1 epidermal growth factor-like repeat (2). The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase (3). The amino acid 2698-3110 encodes the third class A motif cluster in human Megalin, termed Megalin C3. Human Megalin C3 shares 77% and 74% identity with mouse and rat Megalin C3.

References

  1. Christensen, E. I. and Birn, H. (2002) Nat. Rev. Mol. Cell Biol 3:256.
  2. Saito, A. et al. (1994) Proc. Natl. Acad. Sci. U. S. A. 91:9725
  3. Kantarci, S. et al. (2007) Nat. Genet 39:957.

Long Name

LDL Receptor-related Protein 2

Alternate Names

DBS, gp330, LRP-2, LRP2

Entrez Gene IDs

4036 (Human); 14725 (Mouse); 29216 (Rat)

Gene Symbol

LRP2

UniProt

P98164

Additional Megalin/LRP2 Products

Product Documents for Recombinant Human Megalin/LRP-2 (C3) Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Megalin/LRP-2 (C3) Protein, CF

For research use only

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