Recombinant Human Melanotransferrin/CD228 His Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10696-MT

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

NP_005920.2

Conjugate

Unconjugated

Applications

Bioactivity

View all Melanotransferrin/CD228 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Melanotransferrin/CD228 protein
Gly20-Gly711, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly20

Predicted Molecular Mass

76 kDa

SDS-PAGE

84-92 kDa, under reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human Melanotransferrin/CD228 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Human Melanotransferrin/CD228 His Protein, CF

Recombinant Human Melanotransferrin/CD228 His-tag Protein SDS PAGE

2 μg/lane of Recombinant Human Melanotransferrin/CD228 His-tag Protein (Catalog # 10696-MT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-92 kDa and 75-85 kDa, respectively.

Formulation, Preparation and Storage

10696-MT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melanotransferrin/CD228

Melanotransferrin (MTF), also known as CD228, P97, MELTF, or MFI2, is a member of the transferrin superfamily that binds to a single ferric iron with high affinity (1). It was originally identified as a membrane-bound protein with a coding region of 2217 bases encoding 738 amino acids (aa) including five Fe-binding residues, the consensus thermolysin metalloprotease sites, and a glycosyl-phosphatidylinositol (GPI) anchor (2). A secreted form of MTF (sMTF) has also been reported (2, 3). Human MTF shares 86% aa sequence identity with mouse and rat MTF. MTF expression is low in normal tissues but high in tumor and embryonic tissues. In melanoma MTF is associated with tumor metastasis and angiogenesis and upregulated in lung cancer tissue and cell lines (3). In the brain MTF is expressed in capillary endothelium but also in the reactive microglia associated with senile plaques in Alzheimer's disease (AD) (4). sMTF has been reported to be increased in patients with AD or arthritis and may modulate angiogenesis, cell migration, and plasminogen activation (5).

References

  1. Baker, E.N. et al. (1992) FEBS Lett. 298:215.
  2. Sekyere, E. & D.R. Richardson (2000) FEBS Lett. 483:11.
  3. Lei, Y. et al. (2020) Cell Death Dis. 11:933.
  4. Jefferies, W.A. et al. (1996) Brain Res. 712:122.
  5. Paluncic, J. et al. (2016) Biochim. Biophy. Acta. 1863:770.

Alternate Names

CD228, MAP97, MFI2, MTF, p97

Entrez Gene IDs

4241 (Human); 30060 (Mouse); 288038 (Rat)

Gene Symbol

MELTF

UniProt

NP_005920.2

Additional Melanotransferrin/CD228 Products

Product Documents for Recombinant Human Melanotransferrin/CD228 His Protein, CF

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Product Specific Notices for Recombinant Human Melanotransferrin/CD228 His Protein, CF

For research use only

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