Recombinant Human mGluR2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9526-UR

R&D Systems, part of Bio-Techne
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9526-UR-100
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Key Product Details

Source

NS0

Accession #

Q14416

Conjugate

Unconjugated

View all mGluR2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human mGluR2 protein
Gly20-Ser498, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly20

Predicted Molecular Mass

54 kDa

SDS-PAGE

60-75 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9526-UR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR2

Metabotropic glutamate receptors (mGluRs) are coupled to effector systems through GTP-binding proteins and modulate glutamate neurotransmission in the central and peripheral nervous systems (1). Structurally, members from this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane domains, and a cytoplasmic carboxyl-terminal domain variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (3). Intracellularly, a short C-terminus interacts directly with a G-protein (2). The receptors are subdivided into three groups (I–III) based on sequence homology, signal transduction and pharmacological properties (1). The Group II receptors comprise mGLuR2 and mGLuR3, which share high sequence homology. mGLuR2 is a presynaptic receptor expressed on both neurons and glia, and its activation regulates cAMP accumulation (1). Mature human mGluR2 is 854 amino acids in length, including a 549 amino acid (aa) N-terminal ECD. Within N-terminal ECD, human mGluR2 shares 98% aa sequence identity with mouse and rat mGluR2.

References

  1. Conn P.J. and Pin J.P. (1997) Annu. Rev. Pharmacol. Toxicol. 37:205.
  2. Pin J.P. and Duvoisin R. (1995) Neuropharmacology. 34:1.
  3. Niswender C.M. and Conn P.J. (2010) Annu. Rev. Pharmacol. Toxicol. 50:295.

Long Name

Metabotropic Glutamate Receptor 2

Alternate Names

GPRC1B, GRM2, mGluR7

Entrez Gene IDs

2912 (Human); 108068 (Mouse); 24415 (Rat)

Gene Symbol

GRM2

UniProt

Q14416

Additional mGluR2 Products

Product Documents for Recombinant Human mGluR2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human mGluR2 Protein, CF

For research use only

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