Recombinant Human mGluR3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9527-UR

R&D Systems, part of Bio-Techne
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9527-UR-100
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Key Product Details

Source

CHO

Accession #

Q14832-1

Structure / Form

Disulfide linked homodimer

Conjugate

Unconjugated

View all mGluR3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human mGluR3 protein
Asp25-Ser507, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp25

Predicted Molecular Mass

56 kDa

SDS-PAGE

60-74 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9527-UR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR3

Metabotropic glutamate receptors (mGluRs) are coupled to effector systems through GTP-binding proteins and modulate glutamate neurotransmission in the central and peripheral nervous systems (1). Structurally, members from this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane, and a cytoplasmic carboxyl-terminal domain variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (3). Intracellularly, a short C-terminus interacts directly with a G-protein (2). The receptors are subdivided into three groups (I-III) based on sequence homology, signal transduction and pharmacological properties (1). The Group II receptors comprise mGluR2 and mGluR3, which share high sequence homology. mGluR3 is a presynaptic receptor expressed on both neurons and glia, and its activation regulates cAMP accumulation (1). Mature human mGluR3 is 857 amino acids (aa) in length and aa 2-507 represents the N-terminal ECD (4), with the apparent molecular mass approximately 66 kDa in SDS-PAGE under reducing conditions. Over aa 2-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3.

References

  1. Conn PJ, et al. (1997) Annu Rev Pharmacol Toxicol 37:205.
  2. Pin JP, et al. (1995) Neuropharmacology. 34:1.
  3. Niswender CM, et al. (2010) Annu Rev Pharmacol Toxicol 50:295.
  4. Monn, J.A. et al. (2015) J Med Chem 58:7526.

Long Name

Metabotropic Glutamate Receptor 3

Alternate Names

GPRC1C, GRM3

Entrez Gene IDs

2913 (Human); 108069 (Mouse); 24416 (Rat)

Gene Symbol

GRM3

UniProt

Q14832-1

Additional mGluR3 Products

Product Documents for Recombinant Human mGluR3 Protein, CF

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Product Specific Notices for Recombinant Human mGluR3 Protein, CF

For research use only

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