Recombinant Human MIA Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9250-IA

R&D Systems, part of Bio-Techne
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9250-IA-100
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Key Product Details

Source

E. coli

Accession #

Q16674

Conjugate

Unconjugated

Applications

Bioactivity

View all MIA Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human MIA protein
Gly25-Gln131, with an N-terminal Met

Purity

>95%, by SDS-PAGE with silver staining, under reducing conditions.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met, Gly25

Predicted Molecular Mass

12 kDa

SDS-PAGE

10 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Fibronectin Fragment 4 (Catalog # 3624-FN) is immubilized at 1 μg/mL, 100 μL/well, Recombinant Human MIA binds with a typical ED50 of 1-6 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 9250-IA in the following applications:

Scientific Data Images for Recombinant Human MIA Protein, CF

Recombinant Human MIA Protein Bioactivity

Recombinant Human MIA Protein Bioactivity

When Recombinant Human Fibronectin Fragment 4 (Catalog # 3624-FN) is coated at 1 μg/mL, Recombinant Human MIA (Catalog # 9250-IA) binds with a typical ED50 of 1-6 μg/mL.

Formulation, Preparation and Storage

9250-IA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIA

Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15 kDa protein that is secreted as a noncovalent homodimer and is structurally related to OTOR/Otoraplin and MIA-2 (1). Mature human MIA contains a SH3 domain and shares 90% and 92% amino acid sequence identity with mouse and rat MIA, respectively (2). Alternative splicing generates a short isoform that lacks the SH3 domain (3). MIA is widely expressed in developing and regenerating cartilage and in the endothelium and parenchyma of developing lungs (4). MIA disrupts cellular interactions with the extracellular matrix by binding to Integrins  alpha4 beta1 and alpha5 beta1 (5). It competes with Fibronectin fragments for Integrin binding and interferes with Integrin signaling (5). It also functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta 3 induced differentiation into chondrocytes [tscheud]. MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair (7). MIA is up-regulated in several cancers including malignant melanoma, lung adenoma, metastatic oral squamous cell carcinoma, neurofibromatosis type 1 (NF-1)-related tumors, and pancreatic cancer (2, 4, 8-10). It is selectively secreted and internalized from the trailing pole of migrating cells (11, 12). This polarization reduces cellular attachment to the matrix at the trailing pole and contributes to directional tumor cell migration (2, 10, 13, 14).

References

  1. Schmidt, J. et al. (2013) Histol. Histopathol. 28:421.
  2. Blesch, A. et al. (1994) Cancer Res. 54:5695.
  3. Hau, P. et al. (2002) J. Invest. Dermatol. 119:562.
  4. Lin, S. et al. (2008) Dev. Biol. 316:441.
  5. Bauer, R. et al. (2006) J. Biol. Chem. 281:11669.
  6. Tscheudschilsuren, G. et al. (2006) Exp. Cell Res. 312:63.
  7. Schmid, R. et al. (2010) Cell Death Dis. 1:e97.
  8. Marr, D.G. et al. (2004) Int. J. Oncol. 25:105.
  9. Sasahira, T. et al. (2010) Eur. J. Cancer 46:2285.
  10. El Fitori, J. et al. (2005) Cancer Cell Int. 5:3.
  11. Schmidt, J. et al. (2010) Cell Res. 20:1224.
  12. Schmidt, J. and A.K. Bosserhoff (2009) Int. J. Cancer 125:1587.
  13. Poser, I. et al. (2004) Oncogene 23:6115.
  14. Guba, M. et al. (2000) Brit. J. Cancer 83:1216.

Long Name

Melanoma Inhibitory Activity

Alternate Names

CD-RAP

Entrez Gene IDs

8190 (Human); 12587 (Mouse)

Gene Symbol

MIA

UniProt

Q16674

Additional MIA Products

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