Recombinant Human MMP-10 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 910-MP
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro and active forms
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human MMP-10 protein
Tyr18-Cys476
Tyr18-Cys476
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Tyr18
Predicted Molecular Mass
52 kDa (pro) & 43 kDa (active)
SDS-PAGE
58 kDa and 48 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >190 pmol/min/µg, as measured under the described conditions.
The specific activity is >190 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 910-MP in the following applications:
Formulation, Preparation and Storage
910-MP
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl, CaCl2 and Brij-35. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: MMP-10
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain.
Long Name
Matrix Metalloproteinase 10
Alternate Names
MMP10, Stromelysin 2
Entrez Gene IDs
4319 (Human)
Gene Symbol
MMP10
UniProt
Additional MMP-10 Products
Product Documents for Recombinant Human MMP-10 Protein, CF
Product Specific Notices for Recombinant Human MMP-10 Protein, CF
For research use only
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