Recombinant Human MMP-14/MT1-MMP Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 918-MP
Key Product Details
Source
E. coli
Accession #
Structure / Form
Pro and catalytic domains
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
E. coli-derived human MMP-14/MT1-MMP protein
Ala21-Gly284, with a C-terminal 10-His tag
Ala21-Gly284, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala21
Predicted Molecular Mass
31 kDa
SDS-PAGE
31 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010).
The specific activity is >450 pmol/min/µg, as measured under the described conditions.
The specific activity is >450 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
918-MP
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: MMP-14/MT1-MMP
As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alphaV (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (2). Recombinant Human MMP-14 consists of the pro and catalytic domains, which can be activated by treatment with furin as described in the Activity Assay Protocol.
References
- Seike, M. (2003) Cancer Lett. 194:1.
- Sato, H. et al. (1994) Nature 370:61.
Long Name
Matrix Metalloproteinase 14/Membrane Type 1 MMP
Alternate Names
MMP-X1, MMP14, MT-MMP1, MT1-MMP
Entrez Gene IDs
4323 (Human)
Gene Symbol
MMP14
UniProt
Additional MMP-14/MT1-MMP Products
Product Documents for Recombinant Human MMP-14/MT1-MMP Protein, CF
Product Specific Notices for Recombinant Human MMP-14/MT1-MMP Protein, CF
For research use only
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