Recombinant Human/Mouse BMPR-II Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 811-BR
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived BMPR-II protein
| Human BMPR-II (Ala26 - Ile151, with an N-terminal Met) Accession # Q13873 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met & Ser27
Predicted Molecular Mass
41.5 kDa (monomer)
SDS-PAGE
65-75 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse GDF-9 (Catalog # 739-G9) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human/Mouse BMPR‑II Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.1 ‑ 0.5 μg/mL.
Reviewed Applications
Read 1 review rated 5 using 811-BR in the following applications:
Formulation, Preparation and Storage
811-BR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BMPR-II
Like other members of the TGF-beta superfamily, cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the binding to heteromeric complex of type I and type II serine-threonine kinase receptors. Both receptor types are required for the signl transduction. BMP receptor II (BMPR-II) is one of the five mammalian type II receptors (including TGF-beta R-II, ActR-II, ActR-IIB, BMPR-II and MISR-II) for the TGF-beta superfamily ligands. The type II receptors for TGF-beta and activin bind ligands with high affinity by itself. In contrast, BMPR-II binds BMP-2, BMP-4 and BMP-7 weakly in the absence of type I receptor, and the binding can be facilitated by the presence of the type I receptor. BMPR-II mRNA is widely expressed in fetal and adult tissues. Human and mouse BMPR-II are highly conserved sharing 97% sequence identity.
References
- Rosenzweig, B.L. et al. (1995) Proc. Natl. Acad. Sci. USA 92:7632.
- Beppu, H. et al. (1997) Biochem. Biophys. Res. Commun. 235:499.
- Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
Long Name
Bone Morphogenetic Protein Receptor II
Alternate Names
BMPR2, BMPRII
Gene Symbol
BMPR2
UniProt
Additional BMPR-II Products
Product Documents for Recombinant Human/Mouse BMPR-II Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human/Mouse BMPR-II Fc Chimera Protein, CF
For research use only
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