Recombinant Human MTAP His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10379-MT

R&D Systems, part of Bio-Techne
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10379-MT-050
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Key Product Details

Source

E. coli

Accession #

Q13126-1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all MTAP Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human MTAP protein
Ala2-His283
with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

32 kDa

SDS-PAGE

30-32 kDa under reducing conditions

Activity

Measured by its ability to produce adenine through the conversion of 5'methylthioadenine to 5'methylthioribose-1-phosphate.
The specific activity is >650 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human MTAP His-tag Protein, CF

Recombinant Human MTAP His-tag Protein Enzyme Activity

Recombinant Human MTAP His-tag Protein Enzyme Activity

Recombinant Human MTAP His-tag (Catalog # 10379-MT) is measured by its ability to produce adenine through the conversion of 5'methylthioadenine to 5'methylthioribose-1 phosphate.
Recombinant Human MTAP His-tag Protein SDS-PAGE

Recombinant Human MTAP His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human MTAP His-tag (Catalog # 10379-MT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-32 kDa under reducing conditions.

Formulation, Preparation and Storage

10379-MT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: MTAP

Methyl-thioadenosine phosphorylase/MTAP is part of the PNP/MTAP phosphorylase family and catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA), a major byproduct of polyamine synthesis essential for cell growth and proliferation. MTAP also produces most of the free adenine generated in human cells through a salvage pathway and thus couples the purine salvage pathway with polyamine biosynthesis. MTAP forms an active trimer where each identical 32 kDa monomer contains a separate active site (1). Each active site contains three distinct regions required for base-, methylthioribose-, and sulfate/phosphate-binding (1). MTAP is cytosolic and abundantly expressed in normal cells and tissues (2). In contrast, deficient MTAP expression is observed in many types of tumors including lung, bladder, pancreatic, and endometrial cancer (3) due to hyper-methylation gene suppression (4) or gene deletion (3, 5, 6). MTA accumulation leads to an immunosuppressive tumor microenvironment and apoptotic resistance (7-9) and MTAP directly regulates the level of MTA present. MTAP has been reported as a tumor suppressor (6,10) that may also act in a manner that is independent of enzymatic activity (11) through signaling pathways such as the insulin-like growth factor-1 receptor pathway (12). Potential therapeutic strategies to exploit MTAP deficiency in tumors (13,14) or inhibit MTAP in tumors that express MTAP, such as prostate cancer, are under investigation (15).

References

  1. Appleby, T.C. et al. (1999) Structure 7:629.
  2. Garbers, D.L. (1978) Biochim. Biophys. Acta. 523:82.
  3. Li, Y. et al. (2019) J. Cancer 10:927.
  4. Hellerbrand, C. et al. (2006) Carcinogenesis 27:64.
  5. Cairns, P. et al. (1995) Nat. Genet. 11:210.
  6. Christopher, S.A. (2002) Cancer Res. 62:6639.
  7. Kirovski, G. et al. (2011) Am. J. Pathol. 178:1145.
  8. Czech, B. et al. (2013) PLoS One 8:e80703.
  9. Henrich, F.C. et al. (2016) Oncoimmunology 5:e1184802.
  10. Kadariya, Y. et al. (2009) Cancer Res. 69:5961.
  11. Tang, B. et al. (2014) G3 (Bethesda) 5:35.
  12. Xu, J. et al. (2019) Signal Transduct. Target Ther. 4:2.
  13. Bertino, J.R. et al. (2011) Cancer Biol. Ther. 11:627.
  14. Tang, B. et al. (2018) Cancer Res. 78:4386.
  15. Bistulfi, G. et al. (2016) Oncotarget 7:14380.

Long Name

5'-Methylthioadenosine Phosphorylase

Alternate Names

BDMF, C86fus, DMSFH, DMSMFH, HEL-249, LGMBF, MTAPase

Entrez Gene IDs

4507 (Human); 66902 (Mouse)

Gene Symbol

MTAP

UniProt

Q13126-1

Additional MTAP Products

Product Documents for Recombinant Human MTAP His-tag Protein, CF

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Product Specific Notices for Recombinant Human MTAP His-tag Protein, CF

For research use only

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