Recombinant Human MuSK Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9810

Biotinylated
R&D Systems, part of Bio-Techne
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AVI9810-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all MuSK Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human MuSK protein
Human MuSK
(Leu24-Thr495)
Accession # O15146.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu24

Predicted Molecular Mass

80 kDa

SDS-PAGE

87-102 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human MuSK Fc Chimera Avi-tag (Catalog # AVI9810) is immobilized onto a Streptavidin coated plate  (Catalog # CP004) at 2 µg/mL (100 µL/well), in the presence of Recombinant Human Agrin  (Catalog # 6624-AG), it binds to Recombinant Human LRP-4  (Catalog # 5948-LR) with an ED50 of 0.600-4.80 ng/mL.

Scientific Data Images for Recombinant Human MuSK Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human MuSK Fc Chimera Avi-tag Protein Binding Activity.

When Biotinylated Recombinant Human MuSK Fc Chimera Avi-tag Protein (Catalog # AVI9810) is immobilized onto a Streptavidin coated plate (CP004) at 2 µg/mL (100 µL/well), in the presence of Recombinant Human Agrin (6624-AG), it binds to Recombinant Human LRP-4 (5948-LR) with an ED50 of 0.600-4.80 ng/mL.

Biotinylated Recombinant Human MuSK Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human MuSK Fc Chimera Avi-tag Protein (Catalog # AVI9810) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 87-102 kDa and 170-200 kDa, respectively.

Formulation, Preparation and Storage

AVI9810
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MuSK

MuSK (muscle-specific kinase) is a 100 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). It contains a 472 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain (1). Within the ECD, Human MuSK shares 91% and 92% aa sequence identity with mouse MuSK and rat MuSK, respectively. It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). Human MuSK has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail (3). MuSK binds the heparin sulfate proteoglycan agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (4), Wnt ligands (5), biglycan (6), and ColQ (7). Recent studies have showed that MuSK does not bind agrin directly, but enhanced the MuSK-LRP4 interaction (7, 8). When Agrin binds to the N-Terminal region of LRP4, this promotes the association of LRP4 and MuSK, which then stimulates MuSK kinase activity (9, 10). Our Avi-tag Biotinylated human MUSK Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Valenzuela, D.M. et al. (1995) Neuron. 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
  4. Kim, N. et al. (2008) Cell 135:334.
  5. Banerjee, S. et al. (2011) Development 138:3287.
  6. Amenta, A.R. et al. (2012) J Neurosci 32:2324.
  7. Otsuka, K. et al (2015) Scientific Reports. 5:13928.
  8. Zhang, B. et al. (2008) Neuron 60:285.
  9. Zhang, W. et al. (2011) J Biol Chem. 286(47):40624.
  10. Burden, S.J. et al. (2017) CSH Perspectives Biol. 5:a009167.

Long Name

Muscle-specific Receptor Tyrosine Kinase

Alternate Names

EC 2.7.10, EC 2.7.10.1, MGC126323, MGC126324, muscle, skeletal, receptor tyrosine kinase, MuSK, skeletal receptor tyrosine-protein kinase

Entrez Gene IDs

4593 (Human); 18198 (Mouse); 81725 (Rat); 102127677 (Cynomolgus Monkey)

Gene Symbol

MUSK

Additional MuSK Products

Product Documents for Recombinant Human MuSK Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human MuSK Fc Chimera Avi-tag Protein, CF

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