Recombinant Human MuSK His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11421-MK

(HEK293 expressed)
R&D Systems, part of Bio-Techne
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11421-MK-050
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Key Product Details

Source

HEK293

Accession #

O15146.1

Conjugate

Unconjugated

Applications

Bioactivity

View all MuSK Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human MuSK protein
Leu24-Thr495, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu24

Predicted Molecular Mass

53 kDa

SDS-PAGE

58-72 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human MuSK His-tag binds to Recombinant Human LRP-4 Protein (Catalog # 5948-LR) / Recombinant Human Agrin Protein (Catalog # 6624-AG) with a ED50 of 0.300-3.00 μg/mL.

Scientific Data Images for Recombinant Human MuSK His-tag Protein, CF

Recombinant Human MuSK His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human MuSK His-tag Protein (Catalog # 11421-MK) binds to Recombinant Human LRP-4 Protein (5948-LR / Recombinant Human Agrin Protein (6624-AG) with a ED50 of 0.300-3.00 μg/mL.

Recombinant Human MuSK His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human MuSK His-tag Protein (Catalog # 11421-MK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-72 kDa, under reducing conditions.

Formulation, Preparation and Storage

11421-MK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MuSK

Musk (muscle-specific kinase) is a 53 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). It contains a 472 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain (1). Within the ECD, Human Musk shares 91% and 92% aa sequence identity with mouse Musk and rat Musk, respectively. It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). Human Musk has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail (3). Musk binds the heparin sulfate proteoglycan Agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (4), Wnt ligands (5), Biglycan (6), and ColQ (7). Recent studies have showed that Musk does not bind Agrin directly, but enhanced the MuSK-LRP4 interaction (7, 8). When Agrin binds to the N-Terminal region of LRP4, this promotes the association of LRP4 and Musk, which then stimulates Musk kinase activity (9, 10).

References

  1. Valenzuela, D.M. et al. (1995) Neuron. 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
  4. Kim, N. et al. (2008) Cell 135:334.
  5. Banerjee, S. et al. (2011) Development 138:3287.
  6. Amenta, A.R. et al. (2012) J Neurosci 32:2324.
  7. Otsuka, K. et al (2015) Scientific Reports. 5:13928.
  8. Zhang, B. et al. (2008) Neuron 60:285.
  9. Zhang, W. et al. (2011) J Biol Chem. 286(47):40624.
  10. Burden, S.J. et al. (2017) CSH Perspectives Biol. 5:a009167.

Long Name

Muscle-specific Receptor Tyrosine Kinase

Alternate Names

EC 2.7.10, EC 2.7.10.1, MGC126323, MGC126324, muscle, skeletal, receptor tyrosine kinase, MuSK, skeletal receptor tyrosine-protein kinase

Entrez Gene IDs

4593 (Human); 18198 (Mouse); 81725 (Rat); 102127677 (Cynomolgus Monkey)

Gene Symbol

MUSK

UniProt

O15146.1

Additional MuSK Products

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Product Specific Notices for Recombinant Human MuSK His-tag Protein, CF

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