Recombinant Human MXRA8/DICAM Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8939-DM

R&D Systems, part of Bio-Techne
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8939-DM-050
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Key Product Details

Source

CHO

Accession #

Q9BRK3

Conjugate

Unconjugated

Applications

Bioactivity

View all MXRA8/DICAM Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human MXRA8/DICAM protein
His23-Gln341, with a C-terminal 6-his tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His23 & Ser34

Predicted Molecular Mass

36 kDa

SDS-PAGE

38-45 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.
The ED50 for this effect is 2-10 μg/mL.

Formulation, Preparation and Storage

8939-DM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MXRA8/DICAM

DICAM, also known as Limitrin and MXRA8, is an approximately 70 kDa transmembrane adhesion protein that is expressed on the endothelium of many tissues (1). In the vascular system, it is found on pericytes, vascular endothelial cells (EC), and the vessel-contacting feet of astrocytes (2). It is up-regulated on EC by VEGF and functions as a negative regulator of angiogenesis (3). It inhibits VEGF-induced signaling, cell migration, capillary tube formation, and in vivo angiogenesis, and promotes EC apoptosis (3). The interaction of DICAM with Integrin  alphaV beta3 enhances cell adhesion and inhibits the differentiation of osteoclasts (1, 4). Mature human DICAM consists of a 322 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and an 80 aa cytoplasmic domain (2). Within the ECD, human DICAM shares 79% aa sequence identity with mouse and rat DICAM. Alternative splicing generates additional isoforms that lack most of the first Ig-like domain or have a substituted signal peptide.

References

  1. Jung, Y.K. et al. (2008) J. Cell Physiol. 216:603.
  2. Yonezawa, T. et al. (2003) Glia 44:190.
  3. Han, S.-W. et al. (2013) Cardiovasc. Res. 98:73.
  4. Jung, Y.-K. et al. (2012) J. Bone Mineral Res. 27:2024.

Long Name

Matrix-Remodelling Associated 8

Alternate Names

Asp3, DICAM, Limitrin

Entrez Gene IDs

54587 (Human); 74761 (Mouse); 313770 (Rat)

Gene Symbol

MXRA8

UniProt

Q9BRK3

Additional MXRA8/DICAM Products

Product Documents for Recombinant Human MXRA8/DICAM Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human MXRA8/DICAM Protein, CF

For research use only

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