Recombinant Human Napsin A Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8489-NA

R&D Systems, part of Bio-Techne
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8489-NA-050
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Key Product Details

Source

CHO

Accession #

O96009

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Napsin A Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Napsin A protein
Thr25-Gly420, with C-terminal 6-His tag

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr25

Predicted Molecular Mass

44 kDa

SDS-PAGE

40-70 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The specific activity is >12 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8489-NA
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Napsin A

Human NAPSA (also called Napsin A) is a 38 kDa glycosylated aspartic protease (1-3). NAPSA/Napsin A is synthesized as a 420 amino acid (aa) proenzyme containing a signal peptide (up to 25 aa), an activation peptide (38 aa), and a 357 aa mature enzyme. Mature human NAPSA/Napsin A shares 74% and 73% aa sequence identity with mature mouse and rat NAPSA/Napsin A, respectively. The NAPSA/Napsin A proenzyme is expressed in lung epithelial cells and within the kidney (3). Kidney dysfunction is associated with a decrease in NAPSA/Napsin A excretion in urine (1). NAPSA/Napsin A expression is also used as a marker for clear cell ovarian carcinomas, pulmonary adenocarcinomas, and renal cell carcinomas (4-8). NAPSA/Napsin A is involved in processing surfactant protein B in type II pneumocytes (9).

References

  1. Schauer-Vukasinovic, V. et al. (2001) Biochim. Biophys. Acta 1524:51.
  2. Tatnell, P.J. et al. (1998) FEBS Lett. 441:43.
  3. Schauer-Vukasinovic, V. et al. (1999) FEBS Lett. 462:135.
  4. Yamashita, Y. et al. (2014) Mod. Pathol. PMID 24721826
  5. Skirnisdottir, I. et al. (2013) BMC Cancer. 13:524.
  6. Ordonez, N.G. (2012) Adv. Anat. Pathol. 19:66.
  7. Jagirdar, J. (2008) Arch. Pathol. Lab. Med. 132:384.
  8. Ao, M.H. et al. (2014) Hum. Pathol. 45:926.
  9. Brasch, F. et al. (2003) J. Biol. Chem. 278:49006.

Alternate Names

ASP4, KAP, NAPA, NAPSA, Napsin-1, SNAPA, TA01/TA02

Entrez Gene IDs

9476 (Human); 16541 (Mouse); 60575 (Rat)

Gene Symbol

NAPSA

UniProt

O96009

Additional Napsin A Products

Product Documents for Recombinant Human Napsin A Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Napsin A Protein, CF

For research use only

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